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Ratiometric sensing of alkaline phosphatase based on the catalytical activity from Mn-Fe layered double hydroxide nanosheets.
Nanoscale ( IF 6.7 ) Pub Date : 2020-01-08 , DOI: 10.1039/c9nr08769a
Chao Peng 1 , Huanhuan Xing , Yuan Xue , Jin Wang , Jing Li , Erkang Wang
Affiliation  

Two-dimensional (2D) Mn-Fe layered double hydroxide (LDH) nanosheets are firstly examined to mediate an O-phenylenediamine (OPD) based fluorescent switch in the presence of ascorbic acid (AA). On one hand, Mn-Fe LDH mimicked the functions of oxidase to catalyze the oxidation of OPD to OPDox, emitting fluorescence at 565 nm. On the other hand, Mn-Fe LDH acts as a superior catalyst for the reaction between AA and OPD to generate 3-(1,2-dihydroxyethyl)furo[3,4-b]quinoxalin-1(3H)-one (N-heterocyclic compound) with an emergence of the maximum emission at 425 nm (ca. 6 fold). The presence of AA not only induces the enhanced emission at 425 nm from the N-heterocyclic compound, but also leads to decreased fluorescence at 565 nm due to the decomposition of Mn-Fe LDH nanosheets. On the basis of the reversed fluorescence response at 425 and 565 nm, ratiometric fluorescence sensing methods (ΔF425 nm/ΔF565 nm) are developed for the determination of AA. With the assistance of alkaline phosphatase (ALP), the activity of ALP can be monitored using the ratiometric platform based on the hydrolyzing ascorbic acid 2-phosphate to yield AA with the detection limit of 0.16 mU mL-1. Different from the traditional ratiometric sensing platform, where two fluorescent probes are often introduced, the present ratiometric system derived from one signal precursor holds great potential in developing a facile platform and broadens the application of 2D nanomaterials in the field of biology.

中文翻译:

基于Mn-Fe层状双氢氧化物纳米片的催化活性,对碱性磷酸酶进行比例感测。

首先,在抗坏血酸(AA)存在的情况下,对二维(2D)Mn-Fe层状双氢氧化物(LDH)纳米片进行了研究,以介导基于O-苯二胺(OPD)的荧光开关。一方面,Mn-Fe LDH模仿了氧化酶的功能,以催化OPD氧化为OPDox,并在565 nm处发出荧光。另一方面,Mn-Fe LDH可作为AA和OPD反应生成3-(1,2-二羟乙基)呋喃[3,4-b]喹喔啉-1(3H)-one(N -杂环化合物)在425 nm(约6倍)处出现最大发射。AA的存在不仅会导致N杂环化合物在425 nm处发射增强,而且还会由于Mn-Fe LDH纳米片的分解而导致565 nm处荧光减弱。根据425和565 nm的反向荧光响应,比率荧光检测方法(ΔF425nm /ΔF565nm)被开发用于测定AA。在碱性磷酸酶(ALP)的辅助下,可以使用基于水解抗坏血酸2-磷酸的比例平台来监测ALP的活性,得到的AA的检出限为0.16 mU mL-1。与通常引入两个荧光探针的传统比率式传感平台不同,本发明的源自一种信号前体的比率式系统在开发便捷的平台方面具有巨大潜力,并扩大了二维纳米材料在生物学领域的应用。ALP的活性可以使用基于水解抗坏血酸2-磷酸酯以产生AA的定量平台进行监测,检测限为0.16 mU mL-1。与通常引入两个荧光探针的传统比率式传感平台不同,本发明的源自一种信号前体的比率式系统在开发便捷的平台方面具有巨大潜力,并扩大了二维纳米材料在生物学领域的应用。ALP的活性可以使用基于水解抗坏血酸2-磷酸酯以产生AA的定量平台进行监测,检测限为0.16 mU mL-1。与通常引入两个荧光探针的传统比率式传感平台不同,本发明的源自一种信号前体的比率式系统在开发便捷的平台方面具有巨大潜力,并扩大了二维纳米材料在生物学领域的应用。
更新日期:2020-01-08
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