Additive manufacturing (AM) or 3D printing is an ideal technology for building flexible, complex, monolithic devices. Organs-on-chips (OOCs) are biomimetic microsystems that recapitulate the crucial structures and functions of human organs. Organ-level activities, mechanics and physiological response can be stimulated and investigated in OOCs. Convergence of AM technology along with OOCs offers a more efficient route for creating complex organ or tissue structures with precise 3D cell patterning, biomaterial heterogeneity and specific functionalities. Here, we focus on the recent advances in the field, specifically in the fabrication modalities, materials and characterization methods, which are commonly employed for OOCs based on 3D bioprinting. We also discuss the most significant potential applications from integrating 3D bioprinting with OOCs, aiming to provide future strategies for more efficient, automated, modularly integrated, and customizable OOCs.

增材制造（AM）或3D打印是构建灵活，复杂，整体式设备的理想技术。芯片上的器官（OOC）是仿生微系统，概括了人体器官的关键结构和功能。在OOC中可以刺激和研究器官水平的活动，力学和生理反应。AM技术与OOC的融合为创建具有精确3D细胞图案，生物材料异质性和特定功能的复杂器官或组织结构提供了更有效的途径。在这里，我们关注于该领域的最新进展，特别是在基于3D生物打印的OOC中常用的制造方式，材料和表征方法。我们还将讨论将3D生物打印与OOC集成在一起的最重要的潜在应用，