This study was conducted to evaluate the expression of fibrinogen receptors on platelets of Philadelphia-negative chronic myeloproliferative neoplasm (MPN) patients. We collected blood samples from 40 consecutive MPN patients and healthy volunteers. We performed flow cytometry analysis of P-selectin expression and integrin beta-3, activation of glycoprotein (GP) IIb/IIIa and fibrinogen receptor exposure (PAC-1 binding). Surprisingly, we found a very low PAC-1 binding capacity in MPN patients; however, the expression of PAC-1 was almost completely recovered with aspirin intake. We hypothesize that the hypercoagulable states observed in MPN patients could depend on a primarily plasma-driven impairment of fibrin turnover and thrombin generation.

中文翻译：

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慢性骨髓增生性肿瘤患者血小板纤维蛋白原受体的异常低表达：阿司匹林会如何改变？

进行这项研究来评估纤维蛋白原受体在费城阴性慢性骨髓增生性肿瘤（MPN）患者的血小板中的表达。我们从40名连续的MPN患者和健康志愿者那里采集了血液样本。我们对P-选择蛋白表达和整联蛋白β-3，糖蛋白（GP）IIb / IIIa激活和纤维蛋白原受体暴露（PAC-1结合）进行了流式细胞仪分析。出人意料的是，我们发现MPN患者中PAC-1的结合能力很低。然而，摄入阿司匹林后，PAC-1的表达几乎完全恢复。我们假设在MPN患者中观察到的高凝状态可能取决于主要由血浆驱动的纤维蛋白更新和凝血酶生成的损害。