PURPOSE Cataracts are the most common eye complications of retinitis pigmentosa (RP). This study aimed to investigate the cytokine profiles of the aqueous humor of RP with cataracts. METHODS The aqueous humor was collected from RP eyes with cataract (RP group, n = 20) and age-related cataract eyes (ARC group, n = 20) during cataract surgery. The levels of 37 mediators were measured with multiplex fluorescent bead-based immunoassay and compared across groups. The correlation among chemokines, growth factors, and cytokines was analyzed with Spearman's rank correlation coefficient. RESULTS Twelve cytokines (IL-1α, IL-1β, IL-4, IL-10, TNF-α, IFN-γ, EGF, GM-CSF, PDGF-AB/BB, TGF-α, BMP-9, and E-selection) were below the limit of detection, and the detection rate of IL-6 was significantly higher in RP group than in the ARC group (P < 0.01). Compared with those in the control group, the aqueous humor levels of monocyte chemoattractant protein-1 (MCP-1), interleukin-(IL-)8, interferon gamma-induced protein (IP)-10, hepatocyte growth factor (HGF), platelet-derived growth factor AA (PDGF-AA), matrix metalloproteinase-2 (MMP-2), MMP3, MMP-7, MMP-8, plasminogen activator inhibitor-1 (PAI-1), and thrombospondin-2 (TSP-2) in the RP group increased significantly (P < 0.01). A lower level of BMP-4 in the aqueous humor was observed in the RP patients than in the controls (P < 0.05). CONCLUSIONS Significantly increased levels of PDGF-AA, MMP2, MMP3, MMP-7, MMP-8, PAI-1, and TSP-2 and lower levels of BMP-4 were found in the aqueous humor of RP patients. This result indicates a disturbance of the extracellular matrix (ECM) and cytokines in RP patients and suggests a possible role of these cytokines in the pathogenesis of capsular contraction syndrome (CCS) in RP patients.

中文翻译：

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色素性视网膜炎患者房水多细胞因子分析

目的 白内障是视网膜色素变性 (RP) 最常见的眼部并发症。本研究旨在调查患有白内障的 RP 房水的细胞因子谱。方法从白内障手术期间患有白内障的RP眼（RP组，n = 20）和年龄相关性白内障眼（ARC组，n = 20）中收集房水。使用基于多重荧光珠的免疫测定法测量了 37 种介质的水平，并在各组之间进行了比较。趋化因子、生长因子和细胞因子之间的相关性用斯皮尔曼等级相关系数进行分析。结果 12 种细胞因子（IL-1α、IL-1β、IL-4、IL-10、TNF-α、IFN-γ、EGF、GM-CSF、PDGF-AB/BB、TGF-α、BMP-9 和 E -selection)均低于检出限，RP组IL-6检出率显着高于ARC组(P < 0.01)。与对照组相比，房水中单核细胞趋化蛋白-1（MCP-1）、白细胞介素-（IL-）8、干扰素γ诱导蛋白（IP）-10、肝细胞生长因子（HGF）、血小板衍生生长因子 AA (PDGF-AA)、基质金属蛋白酶-2 (MMP-2)、MMP3、MMP-7、MMP-8、纤溶酶原激活剂抑制剂-1 (PAI-1) 和血小板反应蛋白-2 (TSP- 2）在RP组中显着增加（P<0.01）。在 RP 患者中观察到的房水中 BMP-4 水平低于对照组（P < 0.05）。结论 RP 患者房水中 PDGF-AA、MMP2、MMP3、MMP-7、MMP-8、PAI-1 和 TSP-2 水平显着增加，BMP-4 水平降低。