Optical imaging is important for understanding brain function. However, established methods with high spatiotemporal resolution are limited by the potential for laser damage to living tissues. We describe an adaptive femtosecond excitation source that only illuminates the region of interest, which leads to a 30-fold reduction in the power requirement for two- or three-photon imaging of brain activity in awake mice for improved high-speed longitudinal neuroimaging.

中文翻译：

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高速多光子显微镜的自适应激发源。

光学成像对于理解脑功能很重要。但是，已建立的具有高时空分辨率的方法受到激光对活体组织的损害的限制。我们描述了一种自适应飞秒激发源，该激发源仅照亮感兴趣的区域，这导致清醒小鼠大脑活动的两光子或三光子成像的功率需求降低了30倍，从而改善了高速纵向神经成像。