Multiciliated cells (MCCs) amplify large numbers of centrioles that convert into basal bodies, which are required for producing multiple motile cilia. Most centrioles amplified by MCCs grow on the surface of organelles called deuterosomes, whereas a smaller number grow through the centriolar pathway in association with the two parent centrioles. Here, we show that MCCs lacking deuterosomes amplify the correct number of centrioles with normal step-wise kinetics. This is achieved through a massive production of centrioles on the surface and in the vicinity of parent centrioles. Therefore, deuterosomes may have evolved to relieve, rather than supplement, the centriolar pathway during multiciliogenesis. Remarkably, MCCs lacking parent centrioles and deuterosomes also amplify the appropriate number of centrioles inside a cloud of pericentriolar and fibrogranular material. These data show that the centriole number is set independently of their nucleation platforms and suggest that massive centriole production in MCCs is a robust process that can self-organize.

中文翻译：

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在多纤毛细胞中没有氘核体的情况下，可能会产生大量的中心粒。

多纤毛细胞 (MCC) 放大大量中心粒，这些中心粒转化为基体，这是产生多根活动纤毛所必需的。大多数由 MCC 扩增的中心粒在称为氘核体的细胞器表面生长，而较少数量的中心粒通过与两个亲本中心粒相关的中心粒通路生长。在这里，我们展示了缺乏氘核体的 MCC 以正常的逐步动力学放大了正确数量的中心粒。这是通过在表面和母中心粒附近大量生产中心粒来实现的。因此，氘核体可能已经进化为在多纤毛发生过程中缓解而不是补充中心粒通路。值得注意的是，缺乏亲本中心粒和氘核体的 MCC 也会在中心粒周围和纤维颗粒物质云中放大适当数量的中心粒。这些数据表明，中心粒数是独立于其成核平台设置的，并表明 MCC 中的大量中心粒生产是一个可以自组织的稳健过程。