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9t18:1 and 11t18:1 activate the MAPK pathway to regulate the expression of PLA2 and cause inflammation in HUVECs.
Food & Function ( IF 6.1 ) Pub Date : 2020-01-02 , DOI: 10.1039/c9fo01982k
Sheng-Ben Hu 1 , Qian Zou , Xin Lv , Ruo-Lin Zhou , Xian Niu , Chen Weng , Fang Chen , Ya-Wei Fan , Ze-Yuan Deng , Jing Li
Affiliation  

trans fatty acids (TFAs) have been reported to promote vascular diseases mainly by promoting apoptosis and inflammation of vascular endothelial cells. However, it has been reported in recent years that elaidic acid (9t18:1) and vaccenic acid (11t18:1) may have different effects on vascular health. This study investigated the effects of 9t18:1 and 11t18:1 on human umbilical vein endothelial cell (HUVEC) function and the possible mechanism of inflammation by analyzing the changes in the phospholipid composition and the relationship between phospholipase A2 (PLA2) and MAPK pathway. Here we found that the effect of 11t18:1 on cell viability, membrane damage and cellular inflammation was significantly lower than that of 9t18:1 (p < 0.05). And 9t18:1 and 11t18:1 had different effects on phospholipid composition. Both 9t18:1 and 11t18:1 significantly increased the protein expression of PLA2. Moreover, the MAPK pathway regulated the expression of PLA2, inflammatory cytokines and cyclooxygenase-2 (COX-2) and the secretion of prostaglandin E2 (PGE2) in HUVECs induced by 9t18:1 and 11t18:1. In conclusion, 9t18:1 and 11t18:1 activated the MAPK pathway which regulated the expression of PLA2 to cause inflammation in HUVECs.

中文翻译:

9t18:1和11t18:1激活MAPK途径来调节PLA2的表达并引起HUVEC的炎症。

据报道,反式脂肪酸(TFA)主要通过促进血管内皮细胞的凋亡和炎症来促进血管疾病。但是,近年来有报道说,桉树酸(9t18:1)和痘苗酸(11t18:1)对血管健康的影响可能不同。本研究通过分析磷脂成分的变化以及磷脂酶A2(PLA2)和MAPK通路之间的关系,研究了9t18:1和11t18:1对人脐静脉内皮细胞(HUVEC)功能的影响以及可能的炎症机制。在这里,我们发现11t18:1对细胞活力,膜损伤和细胞炎症的影响显着低于9t18:1(p <0.05)。9t18:1和11t18:1对磷脂的组成有不同的影响。9t18:1和11t18都:1显着增加PLA2的蛋白表达。此外,MAPK通路调节9t18:1和11t18:1诱导的HUVEC中PLA2,炎性细胞因子和环氧合酶2(COX-2)的表达以及前列腺素E2(PGE2)的分泌。总之,9t18:1和11t18:1激活了MAPK通路,该通路调节PLA2的表达以引起HUVEC炎症。
更新日期:2020-01-02
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