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A colorimetric fluorescent probe for rapid and specific detection of nitrite.
Luminescence ( IF 2.9 ) Pub Date : 2019-12-01 , DOI: 10.1002/bio.3727
Zujun Xu 1 , Wenxiu Shi 1 , Chengjun Yang 1 , Jing Xu 1 , Huapeng Liu 1 , Jing Xu 2 , Baocun Zhu 2
Affiliation  

The method of fluorescent probes has been an important technique for detection of nitrite (NO2 - ). As an important inorganic salt, excessive nitrite would threaten humans and the environment. In this paper, a colorimetric fluorescent probe P-N (1,2-diaminoanthraquinone) with rapid response and high selectivity, which could detect NO2 - by visual colour changes and fluorescence spectroscopy is presented. The probe P-N solution (pH 1) changed from pink to colourless with the addition of NO2 - and fluorescence intensity at 639 nm clearly decreased. Good linear exists between fluorescence intensities and NO2 - concentrations for the range 0-16 μM, and the detection limit was 54 nM (based on a 3σ/slope). Moreover, probe P-N could also detect NO2 - in real water samples, and results were all satisfactory. Probe P-N shows great practical application value for detecting NO2 - in the environment.

中文翻译:

比色荧光探针,用于快速,特异性地检测亚硝酸盐。

荧光探针法一直是检测亚硝酸盐(NO2-)的重要技术。作为重要的无机盐,过量的亚硝酸盐会威胁人类和环境。本文提出了一种比色荧光探针PN(1,2-二氨基蒽醌),具有快速响应和高选择性,可以通过视觉颜色变化和荧光光谱法检测NO2-。探针PN溶液(pH 1)在添加NO2的情况下从粉红色变为无色,并且在639 nm处的荧光强度明显降低。在0-16μM范围内,荧光强度与NO2-浓度之间存在良好的线性关系,检出限为54 nM(基于3σ/斜率)。而且,探针PN也可以检测真实水样中的NO2-,结果均令人满意。
更新日期:2020-03-02
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