当前位置: X-MOL 学术Comp. Biochem. Physiol. A Mol. Integr. Physiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Effect of salinity on the synthesis and concentration of glycine betaine in osmoregulatory tissues from juvenile shrimps Litopenaeus vannamei.
Comparative Biochemistry and Physiology A: Molecular & Integrative Physiology ( IF 2.3 ) Pub Date : 2019-11-29 , DOI: 10.1016/j.cbpa.2019.110628
María F Delgado-Gaytán 1 , Silvia Gómez-Jiménez 1 , Luis A Gámez-Alejo 1 , Jesús A Rosas-Rodríguez 2 , Ciria G Figueroa-Soto 1 , Elisa M Valenzuela-Soto 1
Affiliation  

In marine animals, glycine betaine is one of the main osmolytes accumulated under osmotic stress conditions; nevertheless, in penaeids, shrimps little is known about the pathways involved in glycine betaine biosynthesis. In animal cells, glycine betaine is synthesized by the enzyme betaine aldehyde dehydrogenase (BADH). We herein investigated the salinity effect on the synthesis and concentration of glycine betaine on white shrimp Litopenaeus vannamei. Shrimps were subjected to 10, 20, 35, 40, 50, and 60 ppt salinity conditions for seven days. BADH activity increased in hepatopancreas and gills of shrimps subjected to salinities above 35 ppt salinity. In muscle, the BADH activity decreased at 35 ppt salinity. In hepatopancreas from shrimps subjected to 50 and 60 ppt salinities, BADH activity increased 1.1 and 1.7-fold. At 60 ppt salinity, BADH activity increased 1.5-fold respect to 35 ppt in gills. Glycine betaine concentration increased in hepatopancreas, gills, muscle, and hemolymph in shrimps subjected to salinities above 35 ppt. Glycine betaine concentration also increased at 20 ppt salinity, while at 10 ppt, not detected significant differences. The catch of glycine betaine from hemolymph by the cell likely is carried out to avoid protein denaturalization. Ammonia concentration in the aquarium's water only increased at salinities of 20 ppt and 10 ppt (1.1-fold relative to 35 ppt). Our data demonstrated that in L. vannamei, salinity regulates BADH activity and glycine betaine content in a tissue-specific manner.

中文翻译:

盐度对凡纳滨对虾幼虾渗透调节组织中甘氨酸甜菜碱合成和浓度的影响。

在海洋动物中,甘氨酸甜菜碱是在渗透胁迫条件下积累的主要渗透物之一。然而,在对虾中,虾对甘氨酸甜菜碱生物合成所涉及的途径知之甚少。在动物细胞中,甘氨酸甜菜碱是由甜菜碱醛脱氢酶(BADH)合成的。我们在本文中研究了盐度对南美白对虾凡纳滨对虾甘氨酸甜菜碱的合成和浓度的影响。将虾置于10、20、35、40、50和60 ppt的盐度条件下,持续7天。盐度高于35 ppt的虾的肝胰脏和s中BADH活性增加。在肌肉中,盐度为35 ppt时,BADH活性降低。在受到50和60 ppt盐度的虾的肝胰腺中,BADH活性增加了1.1和1.7倍。在60 ppt的盐度下,BADH活性相对于35的35 ppt增加了1.5倍。盐度高于35 ppt的虾的肝胰腺,腮,肌肉和淋巴中的甘氨酸甜菜碱浓度增加。盐度为20 ppt时,甘氨酸甜菜碱浓度也增加,而在10 ppt时,未发现显着差异。可能是通过细胞从淋巴中捕获甘氨酸甜菜碱来避免蛋白质变性。水族馆水中的氨浓度仅在盐度为20 ppt和10 ppt时增加(相对于35 ppt的1.1倍)。我们的数据表明,在南美白对虾中,盐度以组织特异性方式调节BADH活性和甘氨酸甜菜碱含量。盐度高于35 ppt的虾中的血淋巴和血淋巴。盐度为20 ppt时,甘氨酸甜菜碱浓度也增加,而在10 ppt时,未发现显着差异。可能是通过细胞从淋巴中捕获甘氨酸甜菜碱来避免蛋白质变性。水族馆水中的氨浓度仅在盐度为20 ppt和10 ppt时增加(相对于35 ppt的1.1倍)。我们的数据表明,在南美白对虾中,盐度以组织特异性方式调节BADH活性和甘氨酸甜菜碱含量。盐度高于35 ppt的虾中的血淋巴和血淋巴。盐度为20 ppt时,甘氨酸甜菜碱浓度也增加,而在10 ppt时,未发现显着差异。可能是通过细胞从淋巴中捕获甘氨酸甜菜碱来避免蛋白质变性。水族馆水中的氨浓度仅在盐度为20 ppt和10 ppt时增加(相对于35 ppt的1.1倍)。我们的数据表明,在南美白对虾中,盐度以组织特异性方式调节BADH活性和甘氨酸甜菜碱含量。s的水仅在盐度为20 ppt和10 ppt时增加(相对于35 ppt的1.1倍)。我们的数据表明,在南美白对虾中,盐度以组织特异性方式调节BADH活性和甘氨酸甜菜碱含量。s的水仅在盐度为20 ppt和10 ppt时增加(相对于35 ppt的1.1倍)。我们的数据表明,在南美白对虾中,盐度以组织特异性方式调节BADH活性和甘氨酸甜菜碱含量。
更新日期:2019-11-30
down
wechat
bug