Yarrowia lipolytica has emerged as a biomanufacturing platform for a variety of industrial applications. It has been demonstrated to be a robust cell factory for the production of renewable chemicals and enzymes for fuel, feed, oleochemical, nutraceutical and pharmaceutical applications. Metabolic engineering of this non-conventional yeast started through conventional molecular genetic engineering tools; however, recent advances in gene/genome editing systems, such as CRISPR–Cas9, transposons, and TALENs, has greatly expanded the applications of synthetic biology, metabolic engineering and functional genomics of Y. lipolytica. In this review we summarize the work to develop these tools and their demonstrated uses in engineering Y. lipolytica, discuss important subtleties and challenges to using these tools, and give our perspective on important gaps in gene/genome editing tools in Y. lipolytica.

中文翻译：

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解脂耶氏酵母的基因编辑和基因调控技术的进展和机遇

解脂耶氏酵母已经成为用于各种工业应用的生物制造平台。事实证明，这是一家强大的电池工厂，可生产用于燃料，饲料，油脂化工，营养保健品和制药业的可再生化学品和酶。这种非常规酵母的代谢工程始于常规的分子遗传工程工具。然而，CRISPR / Cas9，转座子和TALENs等基因/基因组编辑系统的最新进展极大地扩展了解脂耶氏酵母的合成生物学，代谢工程和功能基因组学的应用。在这篇综述中，我们总结了开发这些工具的工作及其在解脂耶氏酵母中的示范应用，并讨论了使用这些工具的重要技巧和挑战，