Based on the large number of deaths of lamprey larvae infected by pathogens in the culture process, the infection response of lamprey larvae to different pathogens was studied. Thus, high-throughput sequencing is firstly used to analyze the lamprey larvae infected by Aeromonas hydrophila, Vibrosplendidus, and Saprolegnia parasitica. A high quality of 338,628,426 Reads were obtained and 203,829 transcripts were assembled. A total of 158,001 Unigenes were annotated by Blast comparison, and a relatively comprehensive transcriptome database of lamprey larvae was established. Based on the transcriptome of lamprey larvae infected by three aquatic pathogens, classification results of COG and GO show that the genes classified into signal transduction mechanism accounted for the largest proportion, and 2316 differentially expressed genes were identified and screened out. After infection by the three pathogens, 16 genes and 19 genes expression were up-regulated and down-regulated, respectively. KEGG pathway analysis of differentially expressed genes showed that lamprey larvae were mainly affected their metabolism and oxidative phosphorylation pathways after infection by two gram-negative bacteria, Vibrosplendidus and Aeromonas hydrophila. As a fungus, Saprolegnia parasitica mainly affected ribosome synthesis in lamprey larvae. The results were validated by detecting the expression levels of 13 DEGs at 24 h after pathogens treatment using Q-PCR. The results provide valuable information for further research into the development of immunity and the innate immune response against pathogen invasion during the artificial propagation of lamprey larvae.

中文翻译：

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比较转录组学分析提供了在七种病原体感染下七lamp幼虫的免疫反应的见解。

基于在培养过程中大量的病原菌感染七lamp鳗幼虫的死亡，研究了七lamp鳗幼虫对不同病原体的感染反应。因此，高通量测序首先被用于分析被嗜水气单胞菌，纤细假单胞菌和寄生腐烂腐殖质感染的七lamp鳗幼虫。获得了338,628,426个读段的高质量，并且装配了203,829个转录本。通过Blast比较对总共158,001个Unigene进行了注释，并建立了一个相对全面的七lamp鳗幼虫转录组数据库。根据受三种水生病原体感染的七lamp鳗幼虫的转录组，COG和GO的分类结果表明，分类为信号转导机制的基因占最大比例，鉴定并筛选出2316个差异表达基因。三种病原体感染后，分别有16个基因和19个基因的表达上调和下调。差异表达基因的KEGG通路分析表明，七lamp鳗幼虫在被两种革兰氏阴性细菌，Vibrosplendidus和嗜水气单胞菌感染后，主要影响其代谢和氧化磷酸化途径。作为一种真菌，Saprolegnia parasitica主要影响七lamp鳗幼虫中核糖体的合成。通过使用Q-PCR检测病原体后24小时的13个DEG的表达水平来验证结果。