Trends in Analytical Chemistry ( IF 13.1 ) Pub Date : 2019-11-30 , DOI: 10.1016/j.trac.2019.115750 Preston T. Snee
Since their development at the turn of the millennium, bright and water-soluble semiconductor quantum dots (QDs) have been applied in analytical chemistry. QDs serve as excellent platforms for ratiometric sensing of biologicals via analyte-dependent manipulation of dipolar energy transfer. Several examples are provided, such as sandwich assays comprised of lanthanide donors and QD acceptors and highly multiplexed sensing schemes that have been realized due to QDs’ supermolecular sizes. However, QDs do not conform to the standard Förster energy transfer mechanism. For example, QDs are excellent donors but poor acceptors when coupled to organic dyes; yet, the same is not true for inorganic phosphors. Furthermore, QDs may reduce or oxidize substrates while engaging in dipolar energy transfer with them. Many of these observations have yet to be explained. Fortunately, optical methods are being developed to untangle these competing mechanisms, which will lead to a complete understanding of QD energy transfer processes.
中文翻译:
半导体量子点FRET:生物分析测定中的能量转移机理
自从它们在千年之交发展以来,明亮的水溶性半导体量子点(QD)已被应用在分析化学中。量子点通过偶极子能量转移的依赖于分析物的操作,为生物的比例感测提供了极好的平台。提供了几个示例,例如由镧系元素供体和QD受体组成的夹心测定,以及由于QD的超分子大小而实现的高度复用的传感方案。但是,量子点不符合标准的Förster能量传输机制。例如,量子点是出色的供体,但与有机染料偶联时受体却很差。然而,无机磷光体并非如此。此外,QD可以还原或氧化基板,同时与基板进行偶极能量转移。这些观察中有许多尚待解释。幸运的是,正在开发光学方法来解开这些竞争机制,这将导致对QD能量转移过程的完全理解。