Elevated application potential of fullerene C60 paved the way to think on its adverse effect when it reaches to biological system and environment. Though fullerenes are insoluble in water, various strategies are employed to make it soluble. Method of solubilization with organic solvents, yield cytotoxic responses both in vitro and in vivo. In this study, dextran was used to stabilize C60 particle. Fourier transformed-infrared spectroscopy (FT-IR) and transition electron microscopy (TEM) were used for characterization and it confirms effective surface stabilization and morphological characteristics. This was followed by various cytotoxicity studies to evaluate its bio-nano interactions. The results of the study suggest that the dextran stabilized C60 nanoparticles (Dex-C60) forms uniform suspension in water and was stable up to 72 h. The C6 glial cell-Dex-C60 interactions indicated that the Dex-C60 nanoparticles penetrate deeper into the cells and cause dose dependent toxic response. The result of the study recommended that Dex-C60 nanoparticles should undergo intensive risk assessment before biomedical applications and should take proper safety measure to avoid its entry to the environment.

中文翻译：

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葡聚糖的毒性稳定了富勒烯 C60 对 C6 神经胶质细胞的影响。

富勒烯C60的应用潜力提升为思考其对生物系统和环境的不利影响铺平了道路。尽管富勒烯不溶于水，但人们采用了各种策略使其可溶。用有机溶剂溶解的方法，在体外和体内产生细胞毒性反应。在本研究中，葡聚糖用于稳定 C60 颗粒。傅里叶变换红外光谱 (FT-IR) 和跃迁电子显微镜 (TEM) 用于表征，它证实了有效的表面稳定性和形态特征。随后进行了各种细胞毒性研究以评估其生物纳米相互作用。研究结果表明，葡聚糖稳定的 C60 纳米粒子 (Dex-C60) 在水中形成均匀的悬浮液，并稳定长达 72 小时。C6 神经胶质细胞-Dex-C60 相互作用表明，Dex-C60 纳米颗粒深入细胞并引起剂量依赖性毒性反应。研究结果建议，Dex-C60 纳米粒子在生物医学应用前应进行深入的风险评估，并应采取适当的安全措施避免其进入环境。