Fungal resistance against frequently used antifungal medicines used for invasive candidiasis and other fungal infections is directing scientist for searching and developing novel antifungal drugs. An endophytic fungal strain Alternaria tenuissima OE7 has been isolated from leaves of Ocimum tenuiflorum L. which showed antifungal activity against numbers of human pathogenic fungi including Trichophyton rubrum, Microsporum gypseum, Aspergillus parasiticus, A. flavus, A. fumigates, Candida albicans and C. tropicalis. Thermostable, non-proteinacious antifungal metabolites produced zones of inhibition against all pathogenic fungi tested. The ethyl acetate extract of the cell free supernatant was found inhibitory to the radial growth and conidial germination of T. rubrum and M. gypseum. It also showed cidal mode of action against C. albicans at a concentration of 1.0 mg/ml. Most interestingly, inhibition of biofilm formation and hyphal development of C. albicans were observed upon treatment with EA fraction at comparatively lower concentrations (100–500 μg/ml). Release of intracellular contents from treated cells of Candida and scanning electron microscopic observation suggested cellular disruptions by antifungal metabolites. Checkerboard study revealed synergy between EA fraction of OE7 (150 μg/ml) and fluconazole (30 μg/ml) with ƩFIC of 0.45. Two active fractions viz. band ‘C’ and band ‘G’ derived after thin layer chromatographic analysis showed inhibitory activity against C. albicans with MIC values of 80 μg/ml and 130 μg/ml respectively. GCMS analysis suggested presence of numbers of compounds in each active fraction. Overall observations attest the prospective role of the isolate OE7 as a potent candidate for the production of antifungal metabolites against human pathogenic fungi.

对用于侵入性念珠菌病和其他真菌感染的常用抗真菌药物的真菌耐药性正在指导科学家寻找和开发新的抗真菌药物。内生真菌菌株孢极细OE7已经从叶中分离圣罗勒L.其显示针对人病原真菌包括的号码的抗真菌活性红色毛癣菌，小孢子gypseum，寄生曲霉，黄曲霉，A.熏蒸，白色念珠菌和C.热带。热稳定的，非蛋白质的抗真菌代谢产物产生了对所有测试的病原真菌的抑制区域。发现无细胞上清液的乙酸乙酯提取物抑制红锥藻和石膏霉菌的径向生长和分生孢子萌发。它也显示出在浓度为1.0 mg / ml时对白色念珠菌具有杀灭作用的模式。最有趣的是，在较低浓度（100-500μg/ ml）的EA馏分处理后，观察到了白色念珠菌生物膜形成和菌丝发育的抑制作用。从念珠菌处理过的细胞中释放细胞内内容物扫描电镜观察表明抗真菌代谢物可破坏细胞。棋盘研究显示OE7的EA分数（150μg/ ml）和氟康唑（30μg/ ml）之间的协同作用，ƩFIC为0.45。两个活性成分即。薄层色谱分析后得出的谱带“ C”和谱带“ G”显示出对白色念珠菌的抑制活性，MIC值分别为80μg/ ml和130μg/ ml。GC MS分析表明，每个活性级分中都存在许多化合物。总体观察证明，分离株OE7作为生产抗人类病原性真菌的抗真菌代谢物的有效候选者，具有潜在的作用。