Pingyangmycin (PYM) and boanmycin (BAM), two individual components of bleomycin (bleomycin A5 and bleomycin A6), are glycopeptide antitumor antibiotics. An efficient procedure for the preparation of PYM and BAM from Streptomyces verticillus var. pingyangensis fermentation broth using macroporous cation-exchange (MCE) resin followed by medium-pressure preparative liquid chromatography (MPLC) based on monodisperse poly(styrene-co-divinylbenzene) (p(st-dvb)) microspheres was investigated in this paper. Nine frequently used MCE resins were screened by static adsorption and desorption to enrich PYM and BAM fromthe fermentation broth, and D157 resin was found to be the most effective. After one run of column-based dynamic adsorption and desorption, the contents of PYM and BAM were increased by factors of 13.8 and 12.1 with recovery yields of 84.21% and 81.47%, respectively. The enriched samples were subjected to MPLC with columns prepacked with the PolyRP 10-300 microspheres. The operational parameters of the MPLC, including the stationary phase and mobile phase compositions, sample/stationary phase ratio, sample loading scale and flow rate, were screened and optimized. The results showed that the separation and purification for PYM and BAM by MPLC were dramatically improved with a mobile phase modifier of 0.15 mol/L ammonium chloride aqueoussolution, a flow rate of 10 mL/min and a sample/stationary phase ratio of 1.0:100 (m/v, g/mL), and PYM and BAM with purities of more than 98.65% and 99.12% were obtained, respectively. The total recoveries of PYM and BAM reached 75.38% and 70.31%. The separation and purification method is simple, efficient, energy-saving, environmentally friendly and suitable for the large-scale preparation of high-purity PYM and BAM from Streptomyces verticillus var. pingyangensis fermentation broth.

中文翻译：

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一种有效的方法，可从变色链霉菌中制备高纯度的平阳霉素和博安霉素。大孔阳离子交换树脂和随后的反相制备色谱法分离平阳发酵液。

平阳霉素（PYM）和博安霉素（BAM）是博来霉素的两个单独成分（博来霉素A5和博来霉素A6），是糖肽类抗肿瘤抗生素。一种有效的方法，用于从变色链霉菌中制备PYM和BAM。本文研究了使用大孔阳离子交换（MCE）树脂和基于单分散聚（苯乙烯-共二乙烯基苯）（p（st-dvb））微球的中压制备液相色谱（MPLC）制备的平阳发酵液。通过静态吸附和解吸筛选了9种常用的MCE树脂，以从发酵液中富集PYM和BAM，发现D157树脂是最有效的。经过一轮基于柱的动态吸附和解吸后，PYM和BAM的含量分别增加了13.8和12.1，回收率达到84。分别为21％和81.47％。富集的样品经过预装了PolyRP 10-300微球的色谱柱进行MPLC处理。筛选并优化了MPLC的操作参数，包括固定相和流动相组成，样品/固定相比，样品上样量和流速。结果表明，使用流动相改性剂0.15 mol / L氯化铵水溶液，流速为10 mL / min和样品/固定相之比为1.0：100时，MPLC对PYM和BAM的分离和纯化得到了显着改善。 （m / v，g / mL），纯度分别为98.65％和99.12％以上的PYM和BAM。PYM和BAM的总回收率分别达到75.38％和70.31％。分离纯化方法简单，高效，节能，环保，适合大规模生产轮状链霉菌的高纯度PYM和BAM。平阳发酵液。