BACKGROUND Plant viruses can affect vector's behaviors in order to enhance viral transmission. Cucurbit chlorotic yellows virus (CCYV) (genus Crinivirus) is an emergent RNA plant virus and is transmitted specifically by biotypes B and Q of tobacco whitefly, Bemisia tabaci (Gennadius), in a semipersistent manner. METHODS We used the electrical penetration graph (EPG) to investigate the effect of CCYV on the feeding behaviors of B. tabaci biotypes B and Q. RESULTS CCYV could affect, both directly and indirectly, the feeding behaviors of B. tabaci to various degrees, depending on biotypes and sexes of the insect. CCYV showed stronger direct effects on biotype Q than on biotype B in terms of increased non-phloem probing and phloem salivation. CCYV increased non-phloem probing and phloem salivation more on females than on males of biotype Q, and increased phloem salivation more on females than on males of biotype B. CCYV had stronger indirect effects, via virus-infested plants, on biotype B than on biotype Q by enhancing phloem sap ingestion and feeding bouts. CCYV increased non-phloem probing and feeding bouts more on males than on females of biotype B, and decreased phloem sap ingestion more on males than on females on biotype Q indirectly. CONCLUSIONS The results clearly indicated that CCYV affects the feeding behaviors of B. tabaci, which may lead to increased ability of the B. tabaci for CCYV transmission.

中文翻译：

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葫芦型绿叶黄病毒直接和间接导致烟粉虱的进食行为发生变化。

背景技术植物病毒可影响载体的行为以增强病毒传播。葫芦绿叶黄化病毒（CCYV）（一种crinivirus）是一种新兴的RNA植物病毒，通过烟粉虱Bemisia tabaci（Gennadius）的生物型B和Q特异性传播，呈半持久性。方法我们使用电渗透图（EPG）来研究CCYV对烟粉虱生物型B和Q的摄食行为的影响。结果CCYV可以直接或间接地影响烟粉虱的摄食行为，在不同程度上，取决于昆虫的生物类型和性别。就增加的非韧皮部探测和韧皮部唾液分泌而言，CCYV对生物型Q的直接影响比对生物型B的更强。CCYV在女性身上比在Q型男性上增加了更多的非韧皮部探测和韧皮部唾液分泌，并且通过增加病毒对植物的摄入和进食，CCYV通过病毒侵染的植物对B型生物体的间接作用要强于对Q型生物体的间接作用。CCYV间接增加了雄性非韧皮部探测和进食次数，而生物型B则比女性更多，而雄性中韧皮部汁液摄入的减少量多于雌性生物型Q。结论结果清楚地表明，CCYV影响了烟粉虱的摄食行为，这可能导致烟粉虱的CCYV传播能力增强。CCYV间接增加了雄性非韧皮部探测和进食次数，而生物型B则比女性更多，而雄性中韧皮部汁液摄入的减少量多于雌性生物型Q。结论结果清楚地表明，CCYV影响了烟粉虱的摄食行为，这可能导致烟粉虱的CCYV传播能力增强。CCYV间接增加了雄性非韧皮部探测和进食次数，而生物型B则比女性更多，而雄性中韧皮部汁液摄入的减少量多于雌性生物型Q。结论结果清楚地表明，CCYV影响了烟粉虱的摄食行为，这可能导致烟粉虱的CCYV传播能力增强。