BACKGROUND Dengue is an important mosquito-borne disease. There is currently only one licensed vaccine for dengue prevention. The vaccine provides higher efficacy in pre-vaccination dengue-seropositive persons but a higher risk of subsequent more severe dengue in dengue-seronegative persons. It is recommended that the dengue vaccine may be given in dengue-seropositive individuals or as mass vaccination without individual pre-vaccination screening in areas where the dengue seroprevalence is > 80% in children aged 9 years. We evaluated a dengue specific immunoglobulin G monoclonal antibody-based capture enzyme-linked immunosorbent assay (MAb-ELISA) in the diagnosis of previous dengue infection using serum samples from the cohort study in Ratchaburi Province, Thailand. METHODS The MAb-ELISA was compared to 70% plaque reduction neutralization test (PRNT70) in 453 serum samples from children aged 3-11 years in Ratchaburi Province, Thailand. RESULTS The sensitivity and specificity of MAb-ELISA at the positive to negative (P/N) ratio cut-off level of > 3 were both 0.91 in the diagnosis of previous dengue infection, compared to PRNT70. The false positivity was mainly in Japanese encephalitis (JE) seropositive subjects. CONCLUSIONS This research provides evidence that MAb-ELISA is useful for dengue seroprevalence study and dengue pre-vaccination screening. JE seropositivity was the major cause of false positive result in the study population.

中文翻译：

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基于单克隆抗体的捕获ELISA诊断先前的登革热感染。

背景技术登革热是一种重要的蚊媒传播疾病。当前只有一种用于登革热预防的许可疫苗。该疫苗在疫苗接种前登革热血清阳性的人中提供更高的功效，但是在登革热血清阴性的人中随后发生更严重登革热的风险更高。建议在9岁以下儿童中登革热血清阳性率> 80％的地区，对登革热血清反应阳性的人或不进行单独的预疫苗筛查的人群进行大规模疫苗接种。我们使用来自泰国叻atch府队列研究的血清样本，评估了基于登革热特异性免疫球蛋白G单克隆抗体的捕获酶联免疫吸附测定（MAb-ELISA），以诊断先前的登革热感染。方法将泰国叻atch府3至11岁儿童的453份血清样本中的MAb-ELISA与70％噬斑减少中和试验（PRNT70​​）进行了比较。结果与PRNT70​​相比，MAb-ELISA在阳性/阴性（P / N）截止水平> 3时的敏感性和特异性均为0.91。假阳性主要发生在日本脑炎（JE）血清反应阳性的受试者中。结论这项研究提供了证据，证明MAb-ELISA可用于登革热血清学研究和登革热预疫苗筛查。JE血清阳性是研究人群假阳性结果的主要原因。结果与PRNT70​​相比，MAb-ELISA在阳性/阴性（P / N）截止水平> 3时的敏感性和特异性均为0.91。假阳性主要发生在日本脑炎（JE）血清反应阳性的受试者中。结论这项研究提供了证据，证明MAb-ELISA可用于登革热血清学研究和登革热预疫苗筛查。JE血清阳性是研究人群假阳性结果的主要原因。结果与PRNT70​​相比，MAb-ELISA在阳性/阴性（P / N）截止水平> 3时的敏感性和特异性均为0.91。假阳性主要发生在日本脑炎（JE）血清反应阳性的受试者中。结论这项研究提供了证据，证明MAb-ELISA可用于登革热血清学研究和登革热预疫苗筛查。JE血清阳性是研究人群假阳性结果的主要原因。