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Identification of microRNAs regulated by tobacco curly shoot virus co-infection with its betasatellite in Nicotiana benthamiana.
Virology Journal ( IF 4.8 ) Pub Date : 2019-11-07 , DOI: 10.1186/s12985-019-1234-5 Jiang Du 1 , Gentu Wu 1 , Zhongpiao Zhou 1 , Jiayuan Zhang 1 , Mingjun Li 1 , Miao Sun 1 , Kairong Jiang 1 , Ling Qing 1
Virology Journal ( IF 4.8 ) Pub Date : 2019-11-07 , DOI: 10.1186/s12985-019-1234-5 Jiang Du 1 , Gentu Wu 1 , Zhongpiao Zhou 1 , Jiayuan Zhang 1 , Mingjun Li 1 , Miao Sun 1 , Kairong Jiang 1 , Ling Qing 1
Affiliation
BACKGROUND
MicroRNAs (miRNAs) are a class of 21-24 nucleotide endogenous non-coding small RNAs that play important roles in plant development and defense responses to biotic and abiotic stresses. Tobacco curly shoot virus (TbCSV) is a monopartite begomovirus, cause leaf curling and plant stunting symptoms in many Solanaceae plants. The betasatellite of TbCSV (TbCSB) induces more severe symptoms and enhances virus accumulation when co-infect the plants with TbCSV.
METHODS
In this study, miRNAs regulated by TbCSV and TbCSB co-infection in Nicotiana benthamiana were characterized using high-throughput sequencing technology.
RESULTS
Small RNA sequencing analysis revealed that a total of 13 known miRNAs and 42 novel miRNAs were differentially expressed in TbCSV and TbCSB co-infected N. benthamiana plants. Several potential miRNA-targeted genes were identified through data mining and were involved in both catalytic and metabolic processes, in addition to plant defense mechanisms against virus infections according to Gene Ontology (GO) analyses. In addition, the expressions of several differentially expressed miRNAs and their miRNA-targeted gene were validated through quantitative real time polymerase chain reaction (qRT-PCR) approach.
CONCLUSIONS
A large number of miRNAs are identified, and their target genes, functional annotations also have been explored. Our results provide the information on N. benthamiana miRNAs and would be useful to further understand miRNA regulatory mechanisms after TbCSV and TbCSB co-infection.
更新日期：2019-11-07
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