Controllable and multiple DNA release is critical in modern gene-based therapies. Current approaches require complex assistant molecules for combined release. To overcome the restrictions on the materials and environment, a novel and versatile DNA release method using a nano-electromechanical (NEMS) hypersonic resonator of gigahertz (GHz) frequency is developed. The micro-vortexes excited by ultra-high frequency acoustic wave can generate tunable shear stress at solid–liquid interface, thereby disrupting molecular interactions in immobilized multilayered polyelectrolyte thin films and releasing embedded DNA strands in a controlled fashion. Both finite element model analysis and experiment results verify the feasibility of this method. The release rate and released amount are confirmed to be well tuned. Owing to the different forces generated at different depth of the films, release of two types of DNA molecules with different velocities is achieved, which further explores its application in combined gene therapy. Our research confirmed that this novel platform based on a nano-electromechanical hypersonic resonator works well for controllable single and multi-DNA release. In addition, the unique features of this resonator such as miniaturization and batch manufacturing open its possibility to be developed into a high-throughput, implantable and site targeting DNA release and delivery system.

中文翻译：

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使用千兆赫纳米机电谐振器从多层聚电解质中可编程地释放多 DNA

可控的多重 DNA 释放对于现代基因疗法至关重要。目前的方法需要复杂的辅助分子来进行组合释放。为了克服材料和环境的限制，开发了一种使用千兆赫（GHz）频率的纳米机电（NEMS）高超声速谐振器的新型多功能DNA释放方法。超高频声波激发的微涡流可以在固液界面产生可调的剪切应力，从而破坏固定化多层聚电解质薄膜中的分子相互作用，并以受控的方式释放嵌入的DNA链。有限元模型分析和实验结果均验证了该方法的可行性。释放速率和释放量确认已调好。由于薄膜不同深度产生的力不同，实现了两种不同速度的DNA分子的释放，进一步探索了其在联合基因治疗中的应用。我们的研究证实，这种基于纳米机电高超声速谐振器的新型平台非常适合可控的单DNA和多DNA释放。此外，该谐振器的独特功能，如小型化和批量制造，使其有可能发展成高通量、可植入和位点靶向的DNA释放和递送系统。