当前位置: X-MOL 学术Immun. Ageing › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Akkermansia muciniphila ameliorates the age-related decline in colonic mucus thickness and attenuates immune activation in accelerated aging Ercc1−/Δ7 mice
Immunity & Ageing ( IF 7.9 ) Pub Date : 2019-03-08 , DOI: 10.1186/s12979-019-0145-z
Benthe van der Lugt 1 , Adriaan A van Beek 2, 3 , Steven Aalvink 4 , Ben Meijer 3 , Bruno Sovran 5 , Wilbert P Vermeij 6, 7 , Renata M C Brandt 6 , Willem M de Vos 4, 8 , Huub F J Savelkoul 3 , Wilma T Steegenga 1 , Clara Belzer 4
Affiliation  

The use of Akkermansia muciniphila as potential therapeutic intervention is receiving increasing attention. Health benefits attributed to this bacterium include an improvement of metabolic disorders and exerting anti-inflammatory effects. The abundance of A. muciniphila is associated with a healthy gut in early mid- and later life. However, the effects of A. muciniphila on a decline in intestinal health during the aging process are not investigated yet. We supplemented accelerated aging Ercc1−/Δ7 mice with A. muciniphila for 10 weeks and investigated histological, transcriptional and immunological aspects of intestinal health. The thickness of the colonic mucus layer increased about 3-fold after long-term A. muciniphila supplementation and was even significantly thicker compared to mice supplemented with Lactobacillus plantarum WCFS1. Colonic gene expression profiles pointed towards a decreased expression of genes and pathways related to inflammation and immune function, and suggested a decreased presence of B cells in colon. Total B cell frequencies in spleen and mesenteric lymph nodes were not altered after A. muciniphila supplementation. Mature and immature B cell frequencies in bone marrow were increased, whereas B cell precursors were unaffected. These findings implicate that B cell migration rather than production was affected by A. muciniphila supplementation. Gene expression profiles in ileum pointed toward a decrease in metabolic- and immune-related processes and antimicrobial peptide production after A. muciniphila supplementation. Besides, A. muciniphila decreased the frequency of activated CD80+CD273− B cells in Peyer’s patches. Additionally, the increased numbers of peritoneal resident macrophages and a decrease in Ly6Cint monocyte frequencies in spleen and mesenteric lymph nodes add evidence for the potentially anti-inflammatory properties of A. muciniphila. Altogether, we show that supplementation with A. muciniphila prevented the age-related decline in thickness of the colonic mucus layer and attenuated inflammation and immune-related processes at old age. This study implies that A. muciniphila supplementation can contribute to a promotion of healthy aging.

中文翻译:

Akkermansia muciniphila 可改善加速衰老 Ercc1−/Δ7 小鼠中与年龄相关的结肠粘液厚度下降并减弱免疫激活

使用 Akkermansia muciniphila 作为潜在的治疗干预措施正受到越来越多的关注。这种细菌对健康的益处包括改善代谢紊乱和发挥抗炎作用。A. muciniphila 的丰富度与生命早期、中期和晚年的健康肠道有关。然而,尚未研究 A. muciniphila 对衰老过程中肠道健康下降的影响。我们给加速衰老的 Ercc1−/Δ7 小鼠补充 A. muciniphila 10 周,并研究了肠道健康的组织学、转录和免疫学方面。长期补充A. muciniphila后,结肠粘液层的厚度增加了约3倍,甚至比补充植物乳杆菌WCFS1的小鼠还要厚得多。结肠基因表达谱表明与炎症和免疫功能相关的基因和通路的表达减少,并表明结肠中 B 细胞的存在减少。补充 A. muciniphila 后,脾脏和肠系膜淋巴结中的总 B 细胞频率没有改变。骨髓中成熟和未成熟 B 细胞的频率增加,而 B 细胞前体不受影响。这些发现表明 B 细胞迁移而不是生产受到 A. muciniphila 补充剂的影响。回肠中的基因表达谱表明补充 A. muciniphila 后代谢和免疫相关过程以及抗菌肽产生减少。此外,A. muciniphila 降低了派尔氏淋巴结中激活的 CD80+CD273− B 细胞的频率。此外,腹膜驻留巨噬细胞数量的增加以及脾脏和肠系膜淋巴结中 Ly6Cint 单核细胞频率的减少为 A. muciniphila 的潜在抗炎特性提供了证据。总而言之,我们发现补充 A. muciniphila 可以防止与年龄相关的结肠粘液层厚度下降,并减轻老年时的炎症和免疫相关过程。这项研究表明,补充 A. muciniphila 有助于促进健康衰老。
更新日期:2020-04-22
down
wechat
bug