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Cytokines for evaluation of chronic inflammatory status in ageing research: reliability and phenotypic characterisation
Immunity & Ageing ( IF 7.9 ) Pub Date : 2019-05-21 , DOI: 10.1186/s12979-019-0151-1 Liselot Koelman 1, 2 , Olga Pivovarova-Ramich 3, 4, 5, 6 , Andreas F H Pfeiffer 3, 4, 5 , Tilman Grune 7 , Krasimira Aleksandrova 1, 2
Immunity & Ageing ( IF 7.9 ) Pub Date : 2019-05-21 , DOI: 10.1186/s12979-019-0151-1 Liselot Koelman 1, 2 , Olga Pivovarova-Ramich 3, 4, 5, 6 , Andreas F H Pfeiffer 3, 4, 5 , Tilman Grune 7 , Krasimira Aleksandrova 1, 2
Affiliation
There is a growing interest in the role of inflammageing for chronic disease development. Cytokines are potent soluble immune mediators that can be used as target biomarkers of inflammageing; however, their measurement in human samples has been challenging. This study aimed to assess the reliability of a pro- and anti-inflammatory cytokine panel in a sample of healthy people measured with a novel electrochemiluminescent multiplex immunoassay platform (Meso Scale Discovery, MSD), and to characterize their associations with metabolic and inflammatory phenotypes. Overall, the majority of cytokines were above the limit of detection (in at least 85.3% of the samples). Cytokines IL-6, IL-8, TNF-α, IL-10, IL-13, and IFN-γ showed overall good to fair reliability (ICC > 0.40), whereas IL-1β, IL-2, IL-4, and IL-12p70 showed poor reliability (ICC < 0.40). The reliability estimates were not substantially influenced by participants’ age, sex, obesity and C-reactive protein (CRP) levels. As expected, cytokine concentrations were elevated with advanced age most pronouncedly for IL-6, IL-8, Il-2, IFN- γ, and TNF-α. No major associations with metabolic phenotypes were observed for most cytokines, with the exception of a positive association between IL-6 and TNF-α with body mass index and CRP (ρ: 0.36; ρ: 0.20; ρ: 0.53; ρ: 0.22, respectively), and IFN-γ and IL-10 with CRP (ρ: 0.23 and ρ: 0.19, respectively). Single measurements of selected cytokines using MSD platform, including IL-6, IL-8, IL-10, IL-13, TNF-α, and IFN-γ have shown to be representative of an individual’s average level over time and could be suitable for use in prospective epidemiological and clinical studies. Such studies are highly warranted to characterize associations of cytokines with phenotypes and diseases associated with ageing.
中文翻译:
用于评估衰老研究中慢性炎症状态的细胞因子:可靠性和表型表征
人们越来越关注炎症在慢性疾病发展中的作用。细胞因子是有效的可溶性免疫介质,可用作炎症的靶标生物标志物;然而,它们在人体样本中的测量一直具有挑战性。本研究旨在评估使用新型电化学发光多重免疫测定平台(Meso Scale Discovery,MSD)测量的健康人样本中促炎和抗炎细胞因子面板的可靠性,并表征它们与代谢和炎症表型的关联。总体而言,大多数细胞因子高于检测限(在至少 85.3% 的样本中)。细胞因子 IL-6、IL-8、TNF-α、IL-10、IL-13 和 IFN-γ 显示总体良好至一般的可靠性(ICC > 0.40),而 IL-1β、IL-2、IL-4、和 IL-12p70 显示出较差的可靠性(ICC < 0. 40)。可靠性估计不受参与者年龄、性别、肥胖和 C 反应蛋白 (CRP) 水平的显着影响。正如预期的那样,细胞因子浓度随着年龄的增长而升高,尤其是 IL-6、IL-8、IL-2、IFN-γ 和 TNF-α。除了 IL-6 和 TNF-α 与体重指数和 CRP 呈正相关(ρ:0.36;ρ:0.20;ρ:0.53;ρ:0.22,分别),以及具有 CRP 的 IFN-γ 和 IL-10(分别为 ρ:0.23 和 ρ:0.19)。使用 MSD 平台对选定的细胞因子(包括 IL-6、IL-8、IL-10、IL-13、TNF-α 和 IFN-γ)进行单次测量已证明可以代表个体随时间推移的平均水平,并且可能适用用于前瞻性流行病学和临床研究。
更新日期:2020-04-22
中文翻译:
用于评估衰老研究中慢性炎症状态的细胞因子:可靠性和表型表征
人们越来越关注炎症在慢性疾病发展中的作用。细胞因子是有效的可溶性免疫介质,可用作炎症的靶标生物标志物;然而,它们在人体样本中的测量一直具有挑战性。本研究旨在评估使用新型电化学发光多重免疫测定平台(Meso Scale Discovery,MSD)测量的健康人样本中促炎和抗炎细胞因子面板的可靠性,并表征它们与代谢和炎症表型的关联。总体而言,大多数细胞因子高于检测限(在至少 85.3% 的样本中)。细胞因子 IL-6、IL-8、TNF-α、IL-10、IL-13 和 IFN-γ 显示总体良好至一般的可靠性(ICC > 0.40),而 IL-1β、IL-2、IL-4、和 IL-12p70 显示出较差的可靠性(ICC < 0. 40)。可靠性估计不受参与者年龄、性别、肥胖和 C 反应蛋白 (CRP) 水平的显着影响。正如预期的那样,细胞因子浓度随着年龄的增长而升高,尤其是 IL-6、IL-8、IL-2、IFN-γ 和 TNF-α。除了 IL-6 和 TNF-α 与体重指数和 CRP 呈正相关(ρ:0.36;ρ:0.20;ρ:0.53;ρ:0.22,分别),以及具有 CRP 的 IFN-γ 和 IL-10(分别为 ρ:0.23 和 ρ:0.19)。使用 MSD 平台对选定的细胞因子(包括 IL-6、IL-8、IL-10、IL-13、TNF-α 和 IFN-γ)进行单次测量已证明可以代表个体随时间推移的平均水平,并且可能适用用于前瞻性流行病学和临床研究。