Micro-computerized tomography (micro-CT) is considered as an innovative non-invasive and high-resolution imaging technology. The current research aims to reconstruct the distribution of preputial vessels in different severity of rat congenital hypospadias model by micro-CT, and to provide an anatomic basis for the selection of preputial vessel pedicle flaps in surgery. Pregnant rats were exposed to finasteride from gestational day 12 to 17. Depending on the position of the urethral meatus, the pups were divided into normal, mild hypospadias and severe hypospadias groups. Six months after birth, the preputial blood vessels were observed in vascular perfusion with Microfil (a silicone-based polymer) and scanned by micro-CT. CTvox and NRecon were utilized to reconstruct 3-dimentional (3D) images. A pathological analysis of the specimen was taken in order to determine the position of Microfil. The normal group and the mild hypospadias group had similar preputial image characteristics. At the junction of the inner and outer prepuce, the deep layer vessels of the superficial fascia were transversely distributed and formed a vascular ring-like structure. Among the severe hypospadias group, five had sufficient blood circulation while six had insufficient blood circulation. In sufficient blood circulation type, the ring-like vessels were found at the junction of the inner and outer prepuce similar to that of the normal and mild hypospadias group. However, only a small amount of capillary supply to this area in the insufficient type. The junction of the inner and outer prepuce with abundant blood circulation was suitable to be a vascular pedicle flap. The tubularized preputial island flaps were consistent with the ring-like vessels area, and the original blood supply was retained to the greatest extent.

中文翻译：

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大鼠先天性尿道下裂模型中不同严重程度的牙周血管分布：微计算机断层摄影术的影像学研究

微型计算机断层扫描（micro-CT）被认为是一种创新的无创高分辨率成像技术。目前的研究旨在通过微CT重建不同严重程度的大鼠先天性尿道下裂模型中的皮齿血管分布，并为手术中皮膜齿蒂的选择提供解剖学基础。妊娠大鼠从妊娠第12天到第17天暴露于非那雄胺。根据尿道口的位置，将幼崽分为正常，轻度尿道下裂和严重尿道下裂组。出生六个月后，用Microfil（一种有机硅基聚合物）在血管灌注中观察了皮膜上皮血管，并用micro-CT进行了扫描。CTvox和NRecon用于重建3维（3D）图像。为了确定Microfil的位置，对标本进行了病理分析。正常组和轻度尿道下裂组具有相似的牙周图像特征。在内包膜和外包膜的交界处，浅筋膜的深层血管横向分布并形成血管环状结构。在严重尿道下裂组中，有五个血液循环充分，而六个则血液循环不足。在足够的血液循环类型中，在内侧和外侧包皮的交界处发现了类似于正常和轻度尿道下裂组的环状血管。然而，在不足的类型中，仅少量毛细管供应到该区域。内包皮和外包皮的结合处具有丰富的血液循环，适合作为血管蒂皮瓣。管状的包皮外皮小岛皮瓣与环状血管区域相符，并最大程度地保留了原始血液。