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The genome profiling method can be applied for species identification of biological materials collected at crime scenes.
BMC Genetics ( IF 2.9 ) Pub Date : 2019-06-10 , DOI: 10.1186/s12863-019-0753-9
Takako Kinebuchi 1 , Nozomi Idota 1 , Hajime Tsuboi 1 , Marin Takaso 1 , Risa Bando 1 , Hiroshi Ikegaya 1
Affiliation  

BACKGROUND Various biological materials unrelated to humans are found at crime scenes and it is often important to elucidate the origin of these materials. A genetic locus common to several species is conventionally PCR-amplified with universal primers to identify species. However, not all species can be identified using a single locus. In this study, DNA from 13 commonly handled taxa was analyzed to identify species by a genome profiling (GP) method, which involves random PCR and temperature gradient gel electrophoresis. RESULTS In a clustering analysis, we successfully obtained a single cluster for each species. CONCLUSION The GP method is cost-effective and does not require advanced techniques and knowledge in molecular biology. The random sampling of the whole genome using multiple primers provides substantial genomic information. Therefore, the method is effective for classifying a wide range of species, including animals, plants, and insects, and is useful for crime scene investigations.

中文翻译:

基因组谱分析方法可用于犯罪现场收集的生物材料的物种鉴定。

背景技术在犯罪现场发现了与人类无关的各种生物材料,阐明这些材料的来源通常很重要。通常使用通用引物对几种物种共有的遗传基因座进行PCR扩增,以鉴定物种。但是,并非所有物种都可以使用一个基因座来识别。在这项研究中,通过基因组分析(GP)方法分析了13种常见分类单元的DNA,以鉴定物种,该方法涉及随机PCR和温度梯度凝胶电泳。结果在聚类分析中,我们成功地为每个物种获得了一个聚类。结论GP方法具有成本效益,不需要分子生物学方面的先进技术和知识。使用多种引物对整个基因组进行随机采样可提供大量的基因组信息。所以,
更新日期:2019-06-10
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