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Lipids detection and quantification in oleaginous microorganisms: an overview of the current state of the art
BMC Chemical Engineering Pub Date : 2019-06-05 , DOI: 10.1186/s42480-019-0013-9 Alok Patel , Io Antonopoulou , Josefine Enman , Ulrika Rova , Paul Christakopoulos , Leonidas Matsakas
BMC Chemical Engineering Pub Date : 2019-06-05 , DOI: 10.1186/s42480-019-0013-9 Alok Patel , Io Antonopoulou , Josefine Enman , Ulrika Rova , Paul Christakopoulos , Leonidas Matsakas
Oleaginous microorganisms are among the most promising feedstocks for the production of lipids for biofuels and oleochemicals. Lipids are synthesized in intracellular compartments in the form of lipid droplets. Therefore, their qualitative and quantitative analysis requires an initial pretreatment step that allows their extraction. Lipid extraction techniques vary with the type of microorganism but, in general, the presence of an outer membrane or cell wall limits their recovery. This review discusses the various types of oleaginous microorganisms, their lipid accumulating capabilities, lipid extraction techniques, and the pretreatment of cellular biomass for enhanced lipid recovery. Conventional methods for lipid quantification include gravimetric and chromatographic approaches; whereas non-conventional methods are based on infrared, Raman, nuclear magnetic resonance, and fluorescence spectroscopic analysis. Recent advances in these methods, their limitations, and fields of application are discussed, with the aim of providing a guide for selecting the best method or combination of methods for lipid quantification.
中文翻译:
油脂微生物中的脂质检测和定量:最新技术概述
油生微生物是用于生产生物燃料和油脂化学品的脂质的最有前途的原料之一。脂质以脂质液滴的形式在细胞内区室中合成。因此,它们的定性和定量分析需要一个允许对其进行提取的初始预处理步骤。脂质提取技术随微生物的类型而变化,但通常,外膜或细胞壁的存在限制了它们的回收。这篇综述讨论了各种类型的油脂性微生物,它们的脂质积聚能力,脂质提取技术以及为增强脂质回收率而对细胞生物质进行的预处理。脂质定量的常规方法包括重量法和色谱法。而非常规方法是基于红外,拉曼,核磁共振和荧光光谱分析。讨论了这些方法的最新进展,其局限性和应用领域,旨在为选择最佳方法或方法组合提供指导,以进行脂质定量。
更新日期:2019-06-05
中文翻译:
油脂微生物中的脂质检测和定量:最新技术概述
油生微生物是用于生产生物燃料和油脂化学品的脂质的最有前途的原料之一。脂质以脂质液滴的形式在细胞内区室中合成。因此,它们的定性和定量分析需要一个允许对其进行提取的初始预处理步骤。脂质提取技术随微生物的类型而变化,但通常,外膜或细胞壁的存在限制了它们的回收。这篇综述讨论了各种类型的油脂性微生物,它们的脂质积聚能力,脂质提取技术以及为增强脂质回收率而对细胞生物质进行的预处理。脂质定量的常规方法包括重量法和色谱法。而非常规方法是基于红外,拉曼,核磁共振和荧光光谱分析。讨论了这些方法的最新进展,其局限性和应用领域,旨在为选择最佳方法或方法组合提供指导,以进行脂质定量。