Herein, magnetic porous organic polymers (MOPs) prepared via an azo coupling reaction in water under mild conditions are evaluated for the extraction of seven cardiovascular drugs, including xylocaine, metoprolol, mexiletine, labetalol, propranolol, carvedilol and propafenone, from human urine samples for the first time. The extraction mechanisms are assumed to be electrostatic interaction and π–π stacking. The MOPs exhibited fast adsorption/desorption time (13 min) and high enrichment factors (315–365-fold) for target analytes. Based on this, a method coupling magnetic solid phase extraction with HPLC-UV detection was developed for the analysis of cardiovascular drugs. Under the optimized conditions, the LODs (S/N = 3) were in the range of 0.06–0.57 μg L⁻¹ with an RSD (n = 5) of 4.3–8.6%, and the linear range was 0.2–200 μg L⁻¹ for propranolol, 2–200 μg L⁻¹ for metoprolol, and 0.5–200 μg L⁻¹ for the other five analytes. The developed method was applied for the analysis of cardiovascular drugs in urine samples with recoveries of 89.0–112%. In order to further evaluate the application potential of this method, real-time monitoring of metoprolol in urine samples collected from volunteers treated with metoprolol tablets was performed. The proposed method is simple, fast, and sensitive and has good potential in the monitoring of cardiovascular drugs in competitive sports and clinical analysis.

中文翻译：

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磁性多孔有机聚合物，用于人类尿液样品中心血管药物的提取，然后进行HPLC-UV †

本文对在温和条件下通过偶氮偶合反应在水中制备的磁性多孔有机聚合物（MOP）进行了评估，以从人尿液样品中提取七种心血管药物，包括木卡因，美托洛尔，美西律，拉贝洛尔，普萘洛尔，卡维地洛和普罗帕酮。第一次。假设提取机制为静电相互作用和π–π堆积。MOP对目标分析物表现出快速的吸附/解吸时间（13分钟）和高富集因子（315-365倍）。基于此，开发了一种将磁性固相萃取与HPLC-UV检测相结合的方法，用于分析心血管药物。在最佳条件下，LOD（S / N = 3）在0.06–0.57μgL ^-1范围内，RSD（n= 5）的4.3-8.6％，并且线性范围为0.2-200微克大号^-1普萘洛尔，2-200微克大号^-1为美托洛尔，和0.5-200微克大号^-1为其他五种分析物。该方法用于尿样中心血管药物的分析，回收率为89.0–112％。为了进一步评估该方法的应用潜力，对从接受美托洛尔片剂治疗的志愿者收集的尿液样本中的美托洛尔进行了实时监测。所提出的方法简单，快速，灵敏，在竞技体育和临床分析中对心血管药物的监测具有良好的潜力。