Stanford type A Aortic dissection (TAAD) is a deadly cardiovascular disease but the relationship between inflammatory cytokines and disease pathogenesis is still unclear. Observation of the changes of different chemokines may help to explore the etiology of TAAD much further. Clinical data was collected from TAAD patients (TAAD group) and healthy controls (HC group) in our institute between October 2013 and December 2014. Blood sample was harvested from each subject of two groups. The expression levels of eighty chemokines were examined by protein array technology. Then we tested the expressions of macrophage inflammatory protein 1β (MIP-1β), epithelial neutrophil activating peptide 78 (ENA-78), interleukin 16 (IL-16), interferon inducible protein 10 (IP-10), and FMS-like tyrosine kinase 3 (Flt-3) ligand by using luminex technology. Osteopontin (OPN) and monocyte chemotaxis protein (MCP) levels were analyzed by ELISA kits. The mean age of TAAD group is 49.9 ± 11.2 and 48.7 ± 9.9 in HC group, respectively. 76.0% of TAAD patients and 72.0% of healthy controls were male. MIP-1β and ENA-78 expression in TAAD group were significantly lower than that in HC group, while significant increasing IL-16 level was found. Plasma levels of OPN in TAAD group increased remarkably compared with HC group, but MCP-1 and MCP-2 expression significantly decreased. No correlation was shown between serum CRP levels and plasma level of these cytokines by using Spearman analysis. ROC analysis showed that OPN could be indicators for TAAD diagnosis with sensitivity of 0.92 and specificity of 0.99. Our results provide a reasonable way to focus on the chemokines in understanding the pathogenesis of human TAAD.

中文翻译：

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斯坦福A型主动脉夹层患者趋化因子表达的初步观察

斯坦福 A 型主动脉夹层 (TAAD) 是一种致命的心血管疾病，但炎症细胞因子与疾病发病机制之间的关系仍不清楚。观察不同趋化因子的变化可能有助于进一步探索TAAD的病因。收集2013年10月至2014年12月期间我院TAAD患者（TAAD组）和健康对照组（HC组）的临床资料。采集两组受试者的血液样本。通过蛋白质阵列技术检测了八十种趋化因子的表达水平。然后我们测试了巨噬细胞炎症蛋白 1β (MIP-1β)、上皮中性粒细胞激活肽 78 (ENA-78)、白细胞介素 16 (IL-16)、干扰素诱导蛋白 10 (IP-10) 和 FMS 样酪氨酸的表达激酶 3 (Flt-3) 配体使用 luminex 技术。通过ELISA试剂盒分析骨桥蛋白（OPN）和单核细胞趋化蛋白（MCP）水平。TAAD 组的平均年龄分别为 49.9±11.2 岁和 48.7±9.9 岁，HC 组。76.0% 的 TAAD 患者和 72.0% 的健康对照是男性。TAAD组MIP-1β和ENA-78的表达显着低于HC组，而IL-16水平显着升高。与HC组相比，TAAD组血浆OPN水平显着升高，但MCP-1和MCP-2表达显着降低。使用 Spearman 分析未显示血清 CRP 水平与这些细胞因子的血浆水平之间存在相关性。ROC分析显示OPN可以作为TAAD诊断的指标，灵敏度为0.92，特异度为0.99。