INTRODUCTION The role of immune checkpoint molecules and the tumor immune microenvironment in the development of intracranial germ cell tumors remains unclear. METHODS We investigated the expression of programed cell death-1 (PD-1), programed cell death ligand-1 (PD-L1), and tumor-infiltrating lymphocytes (TILs) in 8 patients who had intracranial germinomas with sufficient tumor tissue by immunohistochemistry, to analyze the associations between their clinical courses and radiological features. The 8 patients were categorized based on the duration between symptom onset and pathological diagnosis into the long-term onset (LTO) group (> 1 year of symptoms) and the short-term onset (STO) group (< 1 year of symptoms). RESULTS Three patients belonged to the LTO group and 5 patients to the STO group. Compared with STO tumors, LTO tumors were significantly associated with a lower ratio of PD-L1-positive tumor cells (p = 0.012), higher number of infiltrating CD3- and CD8-positive lymphocytes (p = 0.016, 0.003, respectively), and lower ratio of PD-1-positive cells per CD8-positive lymphocytes (p = 0.047). LTO germinomas were significantly smaller in size than STO tumors, not associated with hydrocephalus, and tended to be present in patients with older age at diagnosis and atypical tumor location. CONCLUSIONS Our data suggest that the tumor immune microenvironment, including PD-1/PD-L1 signaling, is associated with the growth of intracranial germinomas.

中文翻译：

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肿瘤免疫微环境与颅内生殖瘤的生长有关。

引言免疫检查点分子和肿瘤免疫微环境在颅内生殖细胞肿瘤发展中的作用尚不清楚。方法我们通过免疫组织化学方法研究了8例颅内生殖细胞瘤伴有足够肿瘤组织的患者中程序性细胞死亡1（PD-1），程序性细胞死亡配体1（PD-L1）和肿瘤浸润淋巴细胞（TIL）的表达。 ，以分析其临床过程与放射学特征之间的关联。根据症状发作和病理诊断之间的持续时间将8例患者分为长期发作（LTO）组（症状> 1年）和短期发作（STO）组（症状<1年）。结果LTO组3例，STO组5例。与STO肿瘤相比，LTO肿瘤与较低比例的PD-L1阳性肿瘤细胞（p = 0.012），较高的CD3和CD8阳性浸润淋巴细胞数量（分别为p = 0.016、0.003）和较低的PD-L-比例显着相关每CD8阳性淋巴细胞1阳性细胞（p = 0.047）。LTO生殖瘤的大小显着小于STO肿瘤，与脑积水无关，并且倾向于在诊断和非典型肿瘤位置的年龄较大的患者中出现。结论我们的数据表明，包括PD-1 / PD-L1信号传导在内的肿瘤免疫微环境与颅内生殖瘤的生长有关。每CD8阳性淋巴细胞中PD-1阳性细胞的比例较低（p = 0.047）。LTO生殖瘤的大小显着小于STO肿瘤，与脑积水无关，并且倾向于在诊断和非典型肿瘤位置的年龄较大的患者中出现。结论我们的数据表明，包括PD-1 / PD-L1信号传导在内的肿瘤免疫微环境与颅内生殖瘤的生长有关。每CD8阳性淋巴细胞中PD-1阳性细胞的比例较低（p = 0.047）。LTO生殖瘤的大小显着小于STO肿瘤，与脑积水无关，并且倾向于在诊断和非典型肿瘤位置的年龄较大的患者中出现。结论我们的数据表明，包括PD-1 / PD-L1信号传导在内的肿瘤免疫微环境与颅内生殖瘤的生长有关。