Viruses pose serious infectious disease threats to humans and animals. To significantly decrease the mortality and morbidity caused by virus infections, there is an urgent need of sensitive and rapid point-of-care platforms for virus detection, especially in low-resource settings. Herein, we developed a smartphone-based point-of-care platform for highly sensitive and selective detection of the avian influenza virus based on nanomaterial-enabled colorimetric detection. The 3D nanostructures, which serve as a scaffold for antibody conjugation to capture the avian influenza virus, are made on PDMS herringbone structures with a ZnO nanorod template. After virus capture, the on-chip gold nanoparticle-based colorimetric reaction allows virus detection by naked eyes with a detection limit of 2.7 × 104 EID50/mL, which is one order of magnitude better than that of conventional fluorescence-based ELISA. Furthermore, a smartphone imaging system with data processing capability further improves the detection limit, reaching down to 8 × 103 EID50/mL. The entire virus capture and detection process can be completed in 1.5 h. We envision that this point-of-care microfluidic system integrated with smartphone imaging and colorimetric detection would provide a fast, cheap, sensitive, and user-friendly platform for virus detection in low-resource settings.

中文翻译：

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基于智能手机的即时护理微流平台，该平台配有用于比色病毒检测的ZnO纳米棒模板。

病毒对人类和动物构成严重的传染病威胁。为了显着降低由病毒感染引起的死亡率和发病率，迫切需要灵敏，快速的即时诊断平台进行病毒检测，尤其是在资源匮乏的环境中。在此，我们开发了基于智能手机的即时护理平台，基于基于纳米材料的比色检测技术，可对禽流感病毒进行高度灵敏和选择性的检测。在带有ZnO纳米棒模板的PDMS人字形结构上制作了3D纳米结构，用作抗体偶联捕获禽流感病毒的支架。捕获病毒后，基于金纳米颗粒的片上比色反应可通过肉眼检测病毒，检测极限为2.7×104 EID50 / mL，与传统的基于荧光的ELISA相比，提高了一个数量级。此外，具有数据处理能力的智能手机成像系统进一步提高了检测极限，低至8×103 EID50 / mL。整个病毒捕获和检测过程可以在1.5小时内完成。我们预想，这种集成了智能手机成像和比色检测功能的即时医疗微流控系统将为低资源环境中的病毒检测提供一个快速，廉价，灵敏且用户友好的平台。