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Real-Time Monitoring of Bacteria Clearance From Blood in a Murine Model.
Cytometry Part A ( IF 3.7 ) Pub Date : 2019-11-26 , DOI: 10.1002/cyto.a.23925
Walter N Harrington 1 , Jacqueline Nolan 1 , Dmitry A Nedosekin 1 , Mark S Smeltzer 2 , Vladimir P Zharov 1
Affiliation  

Bloodstream infections, especially those that are antibiotic resistant, pose a significant challenge to health care leading to increased hospitalization time and patient mortality. There are different facets to this problem that make these diseases difficult to treat, such as the difficulty to detect bacteria in the blood and the poorly understood mechanism of bacterial invasion into and out of the circulatory system. However, little progress has been made in developing techniques to study bacteria dynamics in the bloodstream. Here, we present a new approach using an in vivo flow cytometry platform for real‐time, noninvasive, label‐free, and quantitative monitoring of the lifespan of green fluorescent protein‐expressing Staphylococcus aureus and Pseudomonas aeruginosa in a murine model. We report a relatively fast average rate of clearance for S. aureus (k = 0.37 ± 0.09 min−1, half‐life ~1.9 min) and a slower rate for P. aeruginosa (k = 0.07 ± 0.02 min−1, half‐life ~9.6 min). We also observed what appears to be two stages of clearance for S. aureus , while P. aeruginosa appeared only to have a single stage of clearance. Our results demonstrate that an advanced research tool can be used for studying the dynamics of bacteria cells directly in the bloodstream, providing insight into the progression of infectious diseases in circulation. © 2019 International Society for Advancement of Cytometry

中文翻译:

在小鼠模型中实时监测血液中的细菌清除情况。

血流感染,尤其是那些对抗生素产生耐药性的感染,对医疗保健构成重大挑战,导致住院时间和患者死亡率增加。这个问题的不同方面使这些疾病难以治疗,例如难以检测血液中的细菌,以及对细菌入侵和流出循环系统的机制知之甚少。然而,在开发研究血液中细菌动力学的技术方面进展甚微。在这里,我们提出了一种使用体内流式细胞仪平台的新方法,用于实时、无创、无标记和定量监测表达绿色荧光蛋白的金黄色葡萄球菌铜绿假单胞菌的寿命在鼠模型中。我们报告金黄色葡萄球菌的平均清除率相对较快(k = 0.37 ± 0.09 min -1,半衰期约 1.9 分钟),而铜绿假单胞菌的平均清除率则较慢(k = 0.07 ± 0.02 min -1,半衰期)。寿命约 9.6 分钟)。我们还观察到金黄色葡萄球菌铜绿假单胞菌的两个清除阶段。似乎只有一个阶段的清除。我们的研究结果表明,一种先进的研究工具可用于直接研究血液中细菌细胞的动态,从而深入了解循环中传染病的进展。© 2019 国际细胞术进步协会
更新日期:2019-11-26
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