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Morphological Characteristics of Neuronal Death After Experimental Subarachnoid Hemorrhage in Mice Using Double Immunoenzymatic Technique.
Journal of Histochemistry & Cytochemistry ( IF 3.2 ) Pub Date : 2019-09-17 , DOI: 10.1369/0022155419878181
Fumi Nakano 1 , Lei Liu 1 , Fumihiro Kawakita 1 , Hideki Kanamaru 1 , Yoshinari Nakatsuka 1 , Hirofumi Nishikawa 1 , Takeshi Okada 1 , Masato Shiba 1 , Hidenori Suzuki 1
Affiliation  

Subarachnoid hemorrhage (SAH) is a devastating disease. Neuronal death is an important pathophysiology in the acute phase of SAH, but the histopathological features of dying neurons have been poorly studied. Using several staining methods including terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and microtubule-associated protein 2 (MAP-2) double immunolabeling, we investigated the morphological changes of nucleus and cytoskeleton in neurons and sought susceptible areas to neuronal death in filament perforation SAH mice under light microscope. TUNEL and MAP-2 double immunolabeling clearly showed morphological features of shrunken cytoplasm and sometimes curl-like fibers in dying neurons, besides nuclear abnormalities. More dying neurons were detected in the moderate SAH group than in the mild SAH group, and the temporal base cortex was the most susceptible area to neuronal death with deoxyribonucleic acid (DNA) damage among the cerebral cortices and hippocampus at 24 hr after SAH (p<0.01, ANOVA). Lesser hippocampal neuronal death was observed at 24 hr, but neuronal death was significantly increased in the CA1 region at 7 days after SAH (p<0.05, unpaired t-test). Using TUNEL and MAP-2 double immunolabeling, morphological features of not only the nucleus but also the cytoplasm in post-SAH neuronal death with DNA damage can be observed in detail under light microscope:



中文翻译:

使用双重免疫酶技术的实验性蛛网膜下腔出血小鼠神经元死亡的形态学特征。

蛛网膜下腔出血(SAH)是一种破坏性疾病。在SAH急性期,神经元死亡是重要的病理生理学,但是垂死的神经元的组织病理学特征尚未得到很好的研究。使用包括末端脱氧核苷酸转移酶dUTP缺口末端标记(TUNEL)和微管相关蛋白2(MAP-2)双重免疫标记在内的几种染色方法,我们研究了神经元细胞核和细胞骨架的形态变化,并寻找细丝穿孔中神经元死亡的易感区域。 SAH小鼠在光学显微镜下。TUNEL和MAP-2双重免疫标记法清楚地显示了垂死的神经元中细胞质收缩的形态特征,有时还显示出卷曲状纤维,除了核异常。中度SAH组比轻度SAH组中检测到的死亡神经元更多,p <0.01,方差分析)。在24小时观察到较小的海马神经元死亡,但是SAH后7天,CA1区的神经元死亡显着增加(p <0.05,未配对t检验)。使用TUNEL和MAP-2双重免疫标记,可以在光学显微镜下详细观察SAH后神经元死亡并伴有DNA损伤的细胞核以及细胞质的形态特征:

更新日期:2019-11-27
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