Interactions between the genome and the nuclear pore complex (NPC) have been implicated in multiple gene regulatory processes, but the underlying logic of these interactions remains poorly defined. Here, we report high-resolution chromatin binding maps of two core components of the NPC, Nup107 and Nup93, in Drosophila cells. Our investigation uncovered differential binding of these NPC subunits, where Nup107 preferentially targets active genes while Nup93 associates primarily with Polycomb-silenced regions. Comparison to Lamin-associated domains (LADs) revealed that NPC binding sites can be found within LADs, demonstrating a linear binding of the genome along the nuclear envelope. Importantly, we identified a functional role of Nup93 in silencing of Polycomb target genes and in spatial folding of Polycomb domains. Our findings lend to a model where different nuclear pores bind different types of chromatin via interactions with specific NPC sub-complexes, and a subset of Polycomb domains is stabilized by interactions with Nup93.

中文翻译：

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核孔的核心成分结合了染色质的不同状态，并有助于抑制多梳状组织。

基因组与核孔复合体（NPC）之间的相互作用已经牵涉到多个基因调控过程中，但是这些相互作用的潜在逻辑仍然不清楚。在这里，我们报告果蝇细胞中NPC的两个核心组件Nup107和Nup93的高分辨率染色质结合图。我们的研究发现了这些NPC亚基的差异结合，其中Nup107优先靶向活性基因，而Nup93主要与polycomb沉默区相关。与核纤层蛋白相关结构域（LADs）的比较表明，NPC结合位点可以在LADs中发现，这表明基因组沿着核被膜线性结合。重要的是，我们确定了Nup93在Polycomb靶基因沉默和Polycomb域的空间折叠中的功能性作用。