Abstract The research on gaseous VOCs biofilters has often concentrated on process optimization. However, the microbial community change upon operating conditions is not well understood. In this study, three lab-scale biofilters treating gaseous toluene were operated for 66 days with different inocula under changes in inlet loads and nitrogen sources. Three biofilters were inoculated with activated sludge, river sediment or microbial consortia, respectively. The microbial community differed a lot initially but gradually deviated toward similar structures with the same dominant microorganisms, i.e. Proteobacteria, Actinobacteria (phylum level) and Rhodococcus,Pseudomonas(genus level). Among three biofilters, the two biofilters inoculated with activated sludge and river sediment showed higher microbial diversity, better VOCs removal performance and higher metabolic activity. Higher relative abundance of Alcanivorax (3% compared with lower than 0.03%), Pimelobacte (0.05% compared with lower than 0.01%)were detected under low inlet load, and Zoogloea(0.1%), Alkaliphilus(0.2%) were detected when the inlet load was increased. the abundance of Pseudomonasdecreased from 14% to 2% when ammonia was used as nitrogen source instead of nitrate, meanwhile the abundance of Bacillus and Gordoniaincreased from 0.01% to 0.05% and 0.8% to 5.8% respectively. Some special organisms were observed i.e. the intestinal microorganism.

中文翻译：

---

不同接种量、入口负荷和氮源的气相生物过滤器中微生物群落的变化

摘要 气态VOCs生物过滤器的研究往往集中在工艺优化上。然而，微生物群落在操作条件下的变化尚不清楚。在这项研究中，三个实验室规模的生物过滤器处理气态甲苯，在入口负荷和氮源变化的情况下，使用不同的接种物运行 66 天。三个生物过滤器分别接种活性污泥、河流沉积物或微生物聚生体。微生物群落最初差异很大，但逐渐偏离具有相同优势微生物的相似结构，即变形菌属、放线菌属（门级）和红球菌属、假单胞菌属（属级）。在三种生物滤池中，接种活性污泥和河流底泥的两种生物滤池表现出更高的微生物多样性，更好的VOCs去除性能和更高的代谢活性。在低入口负荷下检测到较高的 Alcanivorax（3% 与低于 0.03% 相比）、Pimelobacte（0.05% 与低于 0.01%），以及 Zoogloea（0.1%）、Alkaliphilus（0.2%）入口负荷增加。当以氨代替硝酸盐作为氮源时，假单胞菌的丰度从14%下降到2%，同时芽孢杆菌和戈登菌的丰度分别从0.01%增加到0.05%和0.8%到5.8%。观察到了一些特殊的生物，即肠道微生物。2%) 在入口负载增加时被检测到。当以氨代替硝酸盐作为氮源时，假单胞菌的丰度从14%下降到2%，同时芽孢杆菌和戈登菌的丰度分别从0.01%增加到0.05%和0.8%到5.8%。观察到了一些特殊的生物，即肠道微生物。2%) 在入口负载增加时被检测到。当以氨代替硝酸盐作为氮源时，假单胞菌的丰度从14%下降到2%，同时芽孢杆菌和戈登菌的丰度分别从0.01%增加到0.05%和0.8%到5.8%。观察到了一些特殊的生物，即肠道微生物。