Abstract Biocatalytic processes continue to find increasing application in industry. Therefore enzyme immobilization has also become of increasing importance as a means of allowing enzyme containment within reactors operating in continuous mode or else separation of enzyme after use in (fed-)batch reactors, as well as potential recycle. Whilst much has been reported in the scientific literature about enzyme immobilization methods, in many cases the protocol leads to losses in enzyme activity. In this review we outline the reasons for loss of activity during immobilization and highlight suitable diagnostic tests to elucidate the precise cause and thereby methods to restore activity. The need for standardized reporting of immobilization methods is also emphasized as a means of benchmarking alternative approaches.

中文翻译：

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定义固定化酶制剂所需的参数

摘要 生物催化过程在工业中的应用不断增加。因此，酶固定化也变得越来越重要，作为一种允许在连续模式下操作的反应器内的酶封闭或在（补料）分批反应器中使用后分离酶以及潜在的再循环的手段。虽然在有关酶固定化方法的科学文献中有很多报道，但在许多情况下，该协议会导致酶活性的损失。在这篇综述中，我们概述了在固定过程中失去活动的原因，并强调了合适的诊断测试，以阐明确切的原因，从而恢复活动的方法。还强调需要对固定方法进行标准化报告，作为对替代方法进行基准测试的一种手段。