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Developmental, hormone- and stress-modulated expression profiles of four members of the Arabidopsis copper-amine oxidase gene family.
Plant Physiology and Biochemistry ( IF 6.5 ) Pub Date : 2019-11-25 , DOI: 10.1016/j.plaphy.2019.11.037
Ilaria Fraudentali 1 , Sandip A Ghuge 2 , Andrea Carucci 1 , Paraskevi Tavladoraki 3 , Riccardo Angelini 3 , Renato A Rodrigues-Pousada 4 , Alessandra Cona 3
Affiliation  

Copper-containing amine oxidases (CuAOs) catalyze polyamines (PAs) terminal oxidation producing ammonium, an aminoaldehyde and hydrogen peroxide (H2O2). Plant CuAOs are induced by stress-related hormones, methyl-jasmonate (MeJA), abscisic acid (ABA) and salicylic acid (SA). In the Arabidopsis genome, eight genes encoding CuAOs have been identified. Here, a comprehensive investigation of the expression pattern of four genes encoding AtCuAOs from the α and γ phylogenetic subfamilies, the two peroxisomal AtCuAOα2 (At1g31690) and AtCuAOα3 (At1g31710) and the two apoplastic AtCuAOγ1 (At1g62810) and AtCuAOγ2 (At3g43670), has been carried out by RT-qPCR and promoter::green fluorescent protein-β-glucuronidase fusion (GFP-GUS). Expression in hydathodes of new emerging leaves (AtCuAOγ1 and AtCuAOγ2) and/or cotyledons (AtCuAOα2, AtCuAOγ1 and AtCuAOγ2) as well as in vascular tissues of new emerging leaves and in cortical root cells at the division/elongation transition zone (AtCuAOγ1), columella cells (AtCuAOγ2) or hypocotyl and root (AtCuAOα3) was identified. Quantitative and tissue-specific gene expression analysis performed by RT-qPCR and GUS-staining in 5- and 7-day-old seedlings under stress conditions or after treatments with hormones or PAs, revealed that all four AtCuAOs were induced during dehydration recovery, wounding, treatment with indoleacetic acid (IAA) and putrescine (Put). AtCuAOα2, AtCuAOα3, AtCuAOγ1 and AtCuAOγ2 expression in vascular tissues and hydathodes involved in water supply and/or loss, along with a dehydration-recovery dependent gene expression, would suggest a role in water balance homeostasis. Moreover, occurrence in zones where an auxin maximum has been observed along with an IAA-induced alteration of expression profiles, support a role in tissue maturation and xylem differentiation events.

中文翻译:

拟南芥铜胺氧化酶基因家族的四个成员的发育,激素和应激调节表达谱。

含铜的胺氧化酶(CuAOs)催化多胺(PAs)末端氧化,生成铵,氨基醛和过氧化氢(H2O2)。植物CuAOs由与压力有关的激素,茉莉酸甲酯(MeJA),脱落酸(ABA)和水杨酸(SA)诱导。在拟南芥基因组中,已鉴定出八个编码CuAO的基因。在这里,对来自α和γ系统发育亚科的四个编码AtCuAOs的基因,两个过氧化物酶体AtCuAOα2(At1g31690)和AtCuAOα3(At1g31710)以及两个质外性AtCuAOγ1(At1g62810)和AtCuAOγ2(At3g43670)的表达模式进行了全面研究。通过RT-qPCR和启动子::绿色荧光蛋白-β-葡糖醛酸糖苷酶融合(GFP-GUS)进行。在新出现的叶片(AtCuAOγ1和AtCuAOγ2)和/或子叶(AtCuAOα2,AtCuAOγ1和AtCuAOγ2)以及新出现的叶子的血管组织中以及在分裂/伸长过渡区(AtCuAOγ1)的皮质根细胞中,鉴定出了小柱状细胞(AtCuAOγ2)或下胚轴和根(AtCuAOα3)。在胁迫条件下或在激素或PA处理后,通过RT-qPCR和GUS染色对5日龄和7日龄幼苗进行的定量和组织特异性基因表达分析表明,在脱水恢复,受伤期间,所有四种AtCuAOs均被诱导,吲哚乙酸(IAA)和腐胺(Put)处理。参与供水和/或流失的维管束和水合蛋白中AtCuAOα2,AtCuAOα3,AtCuAOγ1和AtCuAOγ2的表达,以及与脱水恢复有关的基因表达,提示其在水平衡体内平衡中的作用。而且,
更新日期:2019-11-26
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