The interest in inorganic Hg toxicity and carcinogenicity has been pointed to target organs such as kidney, brain or placenta, but only a few studies have focused on the mammary gland. In this work, analytical combination techniques (SDS-PAGE followed by CV-AFS, and nanoUPLC-ESI-MS/MS) were used to determine proteins that could bind Hg in three human mammary cell lines. Two of them were tumorigenic (MCF-7 and MDA-MB-231) and the other one was the non-tumorigenic cell line (MCF-10A). There are no studies that provide this kind of information in breast cell lines with IHg treatment. Previously, we described the viability, uptake and the subcellular distribution of Hg in human breast cells and analysis of RNA-seq about the genes that encode proteins which are related to cytotoxicity of Hg. This work provides important protein candidates for further studies of Hg toxicity in the mammary gland, thus expanding our understanding of how environmental contaminants might affect tumor progression and contribute with future therapeutic methods.

中文翻译：

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用无机汞处理的人乳腺细胞系的金属蛋白质组学分析。

人们对无机汞毒性和致癌性的兴趣集中在肾脏、大脑或胎盘等靶器官上，但只有少数研究集中在乳腺上。在这项工作中，使用分析组合技术（SDS-PAGE、CV-AFS 和 nanoUPLC-ESI-MS/MS）来确定三种人类乳腺细胞系中可结合 Hg 的蛋白质。其中两种是致瘤细胞系（MCF-7 和 MDA-MB-231），另一种是非致瘤细胞系（MCF-10A）。目前还没有研究提供 IHg 治疗的乳腺细胞系的此类信息。之前，我们描述了人类乳腺细胞中汞的活力、摄取和亚细胞分布，以及编码与汞细胞毒性相关的蛋白质的基因的RNA-seq分析。这项工作为进一步研究乳腺汞毒性提供了重要的候选蛋白质，从而扩大了我们对环境污染物如何影响肿瘤进展的理解，并为未来的治疗方法做出了贡献。