In this work, the effects of carbon nanofiber (CNF) dispersed poly-ε-caprolactone (PCL) nanocomposite coatings and biomolecules functionalization on silk fibroin based conducting braided nerve conduits were studied for enhancing Neuro 2a cellular activities. A unique combination of biomolecules (UCM) and varying concentrations of CNF (5, 7.5, 10% w/w) were dispersed in 10% (w/v) PCL solution for coating on degummed silk threads. The coated silk threads were braided to develop the scaffold structure. As the concentration of CNF increased in the coating, the electrical impedance decreased up to 400 Ω indicating better conductivity. The tensile and dynamic mechanical property analysis showed better mechanical properties in CNF coated samples. In vitro cytocompatibility analysis proved the non-toxicity of the developed braided conduits. Cell attachment, growth and proliferation were significantly enhanced on the biomolecule functionalized nanocomposite coated silk braided structure, exhibiting their potential for peripheral nerve regeneration and recovery.

中文翻译：

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纳米复合材料涂层和生物分子功能化对基于丝素蛋白的3D编织支架进行外围神经组织工程的影响。

在这项工作中，研究了碳纳米纤维（CNF）分散的聚ε-己内酯（PCL）纳米复合物涂层和生物分子功能化对基于丝素蛋白的编织神经导管的作用，以增强Neuro 2a细胞的活性。将生物分子（UCM）和不同浓度的CNF（5、7.5、10％w / w）的独特组合分散在10％（w / v）的PCL溶液中，以涂覆在脱胶的丝线上。将涂覆的丝线编织成支架结构。随着涂层中CNF浓度的增加，电阻抗降低至400Ω，表明具有更好的导电性。拉伸和动态力学性能分析显示CNF涂层样品具有更好的力学性能。体外细胞相容性分析证明了所开发的编织导管的无毒性。