Here, we present the characterization of three iPSC lines derived from dermal fibroblasts of old healthy subjects. Fibroblasts were reprogrammed using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could generate the three germ layers (ectoderm, mesoderm and endoderm), maintained a stable karyotype, and were free from Sendai vectors and reprogramming factors. These integration-free iPSCs can serve for establishing control cell cultures in studies searching for phenotypes and mechanisms that could potentially be dysregulated in degenerative diseases.

中文翻译：

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源自老年男性和女性健康受试者的三种无整合iPSC的集合。

在这里，我们介绍了从健康老人的皮肤成纤维细胞衍生的三个iPSC品系的表征。使用编码OCT4，SOX2，KLF4和c-MYC的仙台病毒载体对成纤维细胞进行重编程。iPSC表达内源性多能性标记，可以产生三个胚层（外胚层，中胚层和内胚层），保持稳定的核型，并且不含仙台载体和重编程因子。这些无整合的iPSC可用于研究寻找在退行性疾病中可能失调的表型和机制的研究中建立对照细胞培养物。