Volatile compounds produced by L1 and L8 strains were assayed against mycelia and conidia growth of Monilinia laxa, M. fructicola, M. polystroma, and M. fructigena of stone fruits. Results showed that volatile metabolites inhibited significantly pathogens growth, in particular M. fructigena mycelium growth (70% by L1 and 50% by L8) and M. fructicola conidia germination (85% by L1 and 70% by L8) compared to the control. Moreover, the antagonistic activity was enhanced by the addition of asparagine (120 mg L-1) in the culture media composition. Synthetic pure compounds were tested in vitro on pathogens mycelial and conidia growth and their EC50 values were estimated, confirming 2-phenethyl as the most active compound. For this reason 2-phenethyl and VOCs of both yeast strains were assayed in vivo on cherry, peach, and apricot fruits. Regarding peach fruit, both treatments, yeasts and pure compounds, displayed the best inhibiting action against all the pathogens especially against M. laxa (100% by L1, 84% by L8 and 2-phenethyl). ATR/IR spectroscopy analysis showed how VOCs produced by both strains increase the fruit waxes complexity reducing the pathogens attack so playing an essential role in the antagonistic activity of both yeast strains and on fruit structural composition.

中文翻译：

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金黄色葡萄球菌挥发性有机化合物可作为替代核果核褐变的收获后方法。

测定了L1和L8菌株产生的挥发性化合物对核果的念珠菌，子实果蝇，多基质果蝇和果核果蝇的菌丝体和分生孢子生长的影响。结果表明，与对照相比，挥发性代谢物显着抑制了病原体的生长，特别是果蝇的菌丝体生长（L1占70％，L8占50％）和果蝇分生孢子的萌发（L1占85％，L8占70％）。此外，通过在培养基组合物中添加天冬酰胺（120mg L-1）来增强拮抗活性。在病原体的菌丝体和分生孢子生长上对合成的纯化合物进行了体外测试，并估算了其EC50值，证实了2-苯乙基是活性最高的化合物。由于这个原因，在樱桃，桃和杏果实上对两种酵母菌株的2-苯乙基和VOCs进行了体内分析。对于桃果实，无论是处理方法，酵母还是纯化合物，都对所有病原体，尤其是对松果支原体（对L1的100％，对L8的84％和2-苯乙基）的抑制作用最佳。ATR / IR光谱分析表明，两种菌株产生的VOC如何增加果蜡的复杂性，减少病原体的侵袭，因此在两种酵母菌株的拮抗活性和果实结构组成中都起着至关重要的作用。