Salmonella is a major pathogen having a public health and economic impact in both humans and animals. Six serotypes of the Salmonella genus are mentioned in the Belgian and European regulation as to be rapidly excluded from the food chain (EU regulation N°2160/2003, Belgian royal decree 27/04/2017). The reference method for Salmonella serotyping, including slide-agglutination and biochemical tests, is time-consuming, expensive, not always objective, and therefore does not match the fast identification criteria required by the legislation. In this study, a molecular method, using genetic markers detected by Multiplex Oligonucleotide Ligation - PCR and Luminex technology, was developed for the identification of the 6 Salmonella serotypes and their variants subjected to an official control. The resulting method was validated with the analysis of 971 Salmonella isolated from different matrixes (human, animal, food or environment) and 33 non-Salmonella strains. The results were compared with the reference identifications, achieving an accuracy of 99.7%. The cost-effective high-throughput genoserotyping assay is performed in 1 day and generates objective results, thanks to the automatic interpretation of raw data using a barcode system. In conclusion, it is fully adapted to the implementation in first line laboratories and meets the requirements of the regulation.

中文翻译：

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使用液珠悬浮液测定法对普通沙门氏菌进行基因分型的多重寡核苷酸连接-PCR方法。

沙门氏菌是一种主要的病原体，对人类和动物均具有公共卫生和经济影响。比利时和欧洲法规中提到了沙门氏菌属的六种血清型，它们被迅速排除在食物链之外（欧盟法规N°2160/2003，比利时皇家法令27/04/2017）。沙门氏菌血清分型的参考方法，包括玻片凝集和生化测试，既费时，昂贵，又不总是客观的，因此不符合法规要求的快速鉴定标准。在这项研究中，开发了一种分子方法，该方法使用通过多重寡核苷酸连接法-PCR和Luminex技术检测到的遗传标记来鉴定受官方控制的6种沙门氏菌血清型及其变异。通过对从不同基质（人，动物，食物或环境）和33种非沙门氏菌菌株中分离出的971沙门氏菌进行分析，验证了所得方法的有效性。将结果与参考标识进行了比较，达到了99.7％的准确度。由于使用条形码系统自动解释原始数据，因此经济高效的高通量基因定型分析可在1天之内完成并产生客观结果。总之，它完全适合在一线实验室的实施，并且符合法规的要求。由于使用条形码系统自动解释原始数据，因此经济高效的高通量基因定型分析可在1天之内完成并产生客观结果。总之，它完全适合在一线实验室的实施，并且符合法规的要求。由于使用条形码系统自动解释原始数据，因此经济高效的高通量基因定型分析可在1天之内完成并产生客观结果。总之，它完全适合在一线实验室的实施，并且符合法规的要求。