In the present study, we investigated whether cold plasma activation affected the efficacy of aerosolized hydrogen peroxide against S. Typhimurium and L. innocua. Stem scars and smooth surfaces of grape tomatoes, surfaces of Granny Smith apples and Romaine lettuce (both midrib and upper leaves) and cantaloupe rinds were inoculated with two-strain cocktails of S. Typhimurium and 3-strain cocktails of L. innocua. The inoculated samples were treated with 7.8% aerosolized H2O2 with and without cold plasma for various times. For all fresh produce items and surfaces, cold plasma significantly (P < 0.05) improved the efficacy of aerosolized H2O2 against Salmonella and L. innocua. Without cold plasma activation, H2O2 aerosols only reduced populations of Salmonella by 1.54-3.17 log CFU/piece while H2O2 with cold plasma achieved 2.35-5.50 log CFU/piece reductions of Salmonella. L. innocua was more sensitive to the cold plasma-activated H2O2 than Salmonella. Cold plasma activated H2O2 aerosols reduced Listeria populations by more than 5 log CFU/piece on all types and surfaces of fresh produce except for the tomato stem scar area. Without cold plasma, the reductions by H2O2 were only 1.35-3.77 log CFU/piece. Overall, our results demonstrated that cold plasma activation significantly enhanced the efficacy of H2O2 mist against bacteria on fresh produce.

中文翻译：

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冷血浆增强了雾化过氧化氢在减少葡萄番茄，苹果，哈密瓜和长叶莴苣上的鼠伤寒沙门氏菌和无毒李斯特菌种群中的功效。

在本研究中，我们调查了冷血浆活化是否影响雾化过氧化氢对鼠伤寒沙门氏菌和无毒利什曼原虫的功效。用两株鼠伤寒沙门氏菌和三株无毒李斯特菌混合物接种葡萄番茄的茎疤和光滑表面，格兰尼史密斯苹果和长叶莴苣（中脉和上部叶子）以及哈密瓜果皮。接种样品分别在无冷血浆和无冷血浆的情况下用7.8％雾化过氧化氢处理。对于所有新鲜产品和表面，冷血浆显着（P <0.05）改善了雾化过氧化氢对沙门氏菌和无害利什曼原虫的功效。如果没有冷血浆激活，H2O2气溶胶只会使沙门氏菌种群减少1.54-3.17 log CFU /个，而带有冷血浆的H2O2达到2.35-5。沙门氏菌减少量可减少50 log CFU。与沙门氏菌相比，无害利什曼原虫对冷血浆激活的H2O2更为敏感。冷等离子体活化的H2O2气雾剂在除番茄茎疤区域以外的所有新鲜农产品的类型和表面上，将李斯特菌数量减少了超过5 log CFU /个。在没有冷等离子体的情况下，H2O2的减少仅为1.35-3.77 log CFU /件。总体而言，我们的结果表明，冷等离子体活化显着增强了H2O2薄雾对新鲜农产品上细菌的功效。35-3.77 log CFU /件 总体而言，我们的结果表明，冷等离子体活化显着增强了H2O2薄雾对新鲜农产品上细菌的功效。35-3.77 log CFU /件 总体而言，我们的结果表明，冷等离子体活化显着增强了H2O2薄雾对新鲜农产品上细菌的功效。