This paper reports micropipette resonators, mechanical resonator-integrated micropipettes, which enable selective aspiration and mass measurement of particles or cells suspended in liquids with two orthogonal vibration modes. A custom pipette pulling system is built to provide power-modulated linear heating on a rotating glass capillary to make an asymmetric cross section with extended uniformity.A glass capillary is stretched with the custom puller, cut within the pulled region, polished, mounted on a machined metallic jig, and then coated with a metal. As a result, a doubly clamped tube resonator-integrated micropipette is made. For simultaneous frequency readouts of two orthogonal modes, an optical pickup, originally developed for optical data storage, is configured closely above and properly aligned to the micropipette resonator and two digital phase-locked loops are employed. For mass responsivity calibration, frequency shifts of the micropipette resonator are measured with various liquids and glass microparticles. Buoyant masses of unicellular organisms, Paramecium aurelia, freely swimming in a culture dish are successfully measured with two orthogonal modes.

中文翻译：

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微量移液器共振器可实现目标抽吸和单个颗粒和细胞的质量测量。

本文报道了微型移液器共振器，即集成了机械共振器的微型移液器，它们能够通过两种正交振动模式对悬浮在液体中的颗粒或细胞进行选择性抽吸和质量测量。定制的移液器牵拉系统可在旋转的玻璃毛细管上提供功率调制的线性加热，以形成不对称的横截面，并具有扩展的均匀性。机械加工的金属夹具，然后涂上金属。结果，制成了双重夹持的管谐振器集成的微量移液器。为了同时读取两个正交模式的频率，最初开发用于光学数据存储的光学拾取器，在微移液管谐振器上方紧紧配置并正确对准微移液管谐振器，并采用了两个数字锁相环。对于质量响应率校准，用各种液体和玻璃微粒测量微量移液器谐振器的频移。用两种正交模式成功地测量了在培养皿中自由游动的单细胞生物，金黄色履毛虫的浮游质量。