Insecticides are a key tool in the management of many insect pests of agriculture, including soybean aphids. The selection imposed by insecticide use has often lead to the evolution of resistance by the target pest through enhanced detoxification mechanisms. We hypothesised that exposure of insecticide-susceptible aphids to sublethal doses of insecticides would result in the up-regulation of genes involved in detoxification of insecticides, revealing the genes upon which selection might act in the field. We used the soybean aphid biotype 1 reference genome, version 6.0 as a reference to analyze RNA-Seq data. We identified multiple genes with potential detoxification roles that were up-regulated 12 h after sublethal exposure to esfenvalerate or thiamethoxam. However, these genes were part of a dramatic burst of differential gene expression in which thousands of genes were up- or down-regulated, rather than a defined response to insecticides. Interestingly, the transcriptional burst observed at 12 h s declined dramatically by 24-hrs post-exposure, suggesting a general stress response that may become fine-tuned over time.

中文翻译：

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大豆蚜虫对亚致死性杀虫剂的转录反应。

杀虫剂是管理许多农业虫害（包括大豆蚜虫）的关键工具。通过使用杀虫剂进行选择通常会通过增强的排毒机制导致目标害虫产生抗药性。我们假设，对杀虫剂敏感的蚜虫接触亚致死剂量的杀虫剂会导致参与杀虫剂解毒的基因上调，从而揭示了在田间选择可能起作用的基因。我们使用大豆蚜虫生物型1参考基因组6.0版作为参考来分析RNA-Seq数据。我们鉴定了具有潜在解毒作用的多个基因，这些基因在亚致死性暴露于乙草戊酸或噻虫嗪后12小时被上调。然而，这些基因是差异基因表达急剧爆发的一部分，其中成千上万的基因被上调或下调，而不是对杀虫剂的明确反应。有趣的是，在暴露后24小时，在12小时观察到的转录猝发急剧下降，表明一般的应激反应可能会随着时间的推移而微调。