Abstract Solvents with opposite characteristics in terms of biocompatibility towards Clostridium acetobutylicum and butanol extraction capacity: vegetable oils, 2-butyl-1-octanol and oleyl alcohol were tested in batch extractive fermentations. Concentration profiles of substrate, intermediate and final products were compared with a control (solvent-free) fermentation. By concomitantly removing the inhibitory products, the total initial substrate was completely consumed with both solvents, while about 70% of the substrate was consumed in the solvent-free batch fermentation. Overproduction of butanol attained 60% and 100% with vegetable oil and 2-butyl-1-octanol respectively. Surprisingly, in the case of 2B1O extractive fermentation, a 46% increase of butanol yield (mass of butanol per mass of glucose consumed) was also observed, as well as the doubling of the ratio for the main metabolites (butanol to acetone). However, with this solvent, a longer lag phase compared to solvent free or vegetable oil extractive fermentation was observed, although the extraction capacity of butanol improved significantly due to the higher partition coefficient. Based on these results, differences between solvents go beyond what is expected from thermodynamics (liquid-liquid extraction). Culture metabolism and/or membrane polarity related functions appear to be influenced by the presence of 2B1O. In this context, possible mechanisms for these effects are discussed.

中文翻译：

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使用不同性质的溶剂提取 ABE 发酵中梭菌响应的改变

摘要 在对丙酮丁醇梭菌的生物相容性和丁醇提​​取能力方面具有相反特性的溶剂：在分批提取发酵中测试了植物油、2-丁基-1-辛醇和油醇。将底物、中间产物和最终产物的浓度曲线与对照（无溶剂）发酵进行比较。通过同时去除抑制性产物，两种溶剂都完全消耗了初始底物总量，而在无溶剂分批发酵中消耗了约 70% 的底物。用植物油和 2-丁基-1-辛醇生产的丁醇分别达到 60% 和 100%。令人惊讶的是，在 2B1O 提取发酵的情况下，还观察到丁醇产量（每消耗的葡萄糖质量的丁醇质量）增加了 46%，以及主要代谢物（丁醇与丙酮）的比率加倍。然而，使用这种溶剂，与无溶剂或植物油提取发酵相比，观察到更长的滞后期，尽管由于较高的分配系数，丁醇的提取能力显着提高。基于这些结果，溶剂之间的差异超出了热力学（液-液萃取）的预期。培养物代谢和/或膜极性相关功能似乎受 2B1O 存在的影响。在这种情况下，讨论了这些影响的可能机制。尽管由于分配系数较高，丁醇的萃取能力显着提高。基于这些结果，溶剂之间的差异超出了热力学（液-液萃取）的预期。培养物代谢和/或膜极性相关功能似乎受 2B1O 存在的影响。在这种情况下，讨论了这些影响的可能机制。尽管由于分配系数较高，丁醇的萃取能力显着提高。基于这些结果，溶剂之间的差异超出了热力学（液-液萃取）的预期。培养物代谢和/或膜极性相关功能似乎受 2B1O 存在的影响。在这种情况下，讨论了这些影响的可能机制。