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Investigating the protective actions of D-pinitol against arsenic-induced toxicity in PC12 cells and the underlying mechanism.
Environmental Toxicology and Pharmacology ( IF 4.3 ) Pub Date : 2019-11-21 , DOI: 10.1016/j.etap.2019.103302
Md Shiblur Rahaman 1 , Mahmuda Akter 1 , Md Mostafizur Rahman 2 , Md Tajuddin Sikder 3 , Toshiyuki Hosokawa 4 , Takeshi Saito 5 , Masaaki Kurasaki 6
Affiliation  

Arsenic is awfully toxic metalloid responsible for many human diseases all over the world. Contrastingly, D-pinitol is a naturally occurring bioactive dietary compound has antioxidant properties. The objective of this study is to elucidate the protective actions of D-pinitol on arsenic-induced cytotoxicity and explore its controlling role in biomolecular mechanisms in PC12 cells. Obtained results demonstrated that co-exposure of D-pinitol with arsenic increases cell viability, decreases DNA damage and protects PC12 cells from arsenic-induced cytotoxicity by increasing glutathione (GSH) level and glutathione reductase (GR). Protein expression of western blot analysis showed that co-exposure of D-pinitol and arsenic significantly inhibited arsenic-induced autophagy which further suppressed apoptosis through up-regulation of survival factors; mTOR, p-mTOR, Akt, p-Akt, NF-кB, Nrf2, ERK1, GR, Bcl-x and down-regulation of death factors; p53, Bax, cytochrome c, LC3, although arsenic regulated those factors negatively. These results of this study suggested that D-pinitol protects PC12 cells from arsenic-induced cytotoxicity.



中文翻译:

研究D-松醇对砷诱导的PC12细胞毒性的保护作用及其潜在机制。

砷是剧毒的准金属,可导致全世界许多人类疾病。相反,D-松醇是天然存在的具有抗氧化特性的生物活性饮食化合物。这项研究的目的是阐明D-松醇对砷诱导的细胞毒性的保护作用,并探讨其在PC12细胞生物分子机制中的控制作用。获得的结果表明,通过增加谷胱甘肽(GSH)和谷胱甘肽还原酶(GR)的浓度,砷与D-松醇的共同暴露可提高细胞活力,降低DNA损伤并保护PC12细胞免受砷诱导的细胞毒性作用。Western blot分析的蛋白质表达表明,D-松醇和砷共同暴露可显着抑制砷诱导的自噬,并通过上调生存因子进一步抑制细胞凋亡。mTOR,p-mTOR,Akt,p-Akt,NF-кB,Nrf2,ERK1,GR,Bcl-x和死亡因子的下调;p53,Bax,细胞色素c,LC3,尽管砷对这些因素有不利的调节作用。这项研究的这些结果表明,D-松醇保护PC12细胞免受砷诱导的细胞毒性作用。

更新日期:2019-11-21
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