Abstract Al3+ has no spectroscopic characteristics and its coordination ability is very poor. Therefore, the detection of Al3+ is a challenging task. To address this issue, here we report a diacylhydrazone-based fluorescent chemosensor (L) for Al3+ structurally characterized by HRMS and NMR spectroscopy. The selective test revealed that L had a high selectivity towards Al3+ with a turn-on response in DMF/H2O (v/v, 8: 2) buffer solution. The combination ratio between L and Al3+ was determined to be 1: 2 as supported by Job’s plot analysis and NMR titration experiment. The recognition of Al3+ was without interference of other competing species. The detection limit was calculated to be as low as 2.41 μM. Moreover, the probe was proved to be of low cytotoxicity in physiological condition and it was applied to image Al3+ in living cells successfully.

中文翻译：

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基于二酰腙衍生物的水介质中Al3+选择性开启荧光探针及其细胞成像

摘要 Al3+没有光谱特性，配位能力很差。因此，Al3+ 的检测是一项具有挑战性的任务。为了解决这个问题，我们在这里报告了一种基于二酰基腙的荧光化学传感器 (L)，用于 Al3+ 结构特征的 HRMS 和核磁共振光谱。选择性测试表明，L 对 Al3+ 具有高选择性，在 DMF/H2O (v/v, 8:2) 缓冲溶液中具有开启响应。L 和 Al3+ 之间的组合比被确定为 1:2，由 Job 的绘图分析和 NMR 滴定实验支持。Al3+的识别不受其他竞争物种的干扰。经计算，检测限低至 2.41 μM。此外，该探针在生理条件下被证明具有低细胞毒性，并成功应用于活细胞中的Al3+成像。