RATIONALE Floral volatiles are commonly present only at trace amounts and can be degraded or lost during vapor collection, which is often challenging from the analytical standpoint. Osmanthus fragrans Lour. is a widely cultivated plant known for the highly distinct fragrance of its flowers. The identification of specific volatile organic compounds (VOCs) and molecular differentiation of O. fragrans without any chemical pretreatment and VOC collection are important. METHODS Twenty-eight VOCs released by the flowers from ten different cultivars of O. fragrans were identified using neutral desorption extractive atmospheric pressure chemical ionization mass spectrometry (ND-EAPCI-MS) without any chemical pretreatment or VOC collection. Chemical identification was performed by high-resolution MSn analysis and whenever possible was confirmed by the analysis of standards. RESULTS According to our literature search, nine of the identified VOCs, 3-buten-2-one, cyclohexadiene, 2-methylfuran, 3-allylcyclohexene, cuminyl alcohol, hotrienol oxide, epoxy-linalool oxide, N-(2-hydroxyethyl) octanamide, and 3-hydroxy-dihydro-β-ionone, have not been reported in O. fragrans in earlier studies. Confident differentiation between ten different cultivars of O. fragrans was achieved by the principal component analysis of the mass spectrometric results. CONCLUSIONS The results of our ND-EAPCI-MS analysis substantially increase our knowledge about the chemistry of the O. fragrans floral fragrance and demonstrate the power of this technique for direct molecular profiling for plant recognition or in biotechnological applications.

中文翻译：

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中性解吸萃取大气压化学电离质谱法鉴定桂花中的挥发物并进行分子鉴别。

理由花香挥发物通常仅以痕量存在，并且在蒸气收集过程中会降解或损失，这从分析的角度来看通常是有挑战性的。桂花桂花。是一种广泛种植的植物，以其花朵的高度清香而闻名。未经化学预处理和VOC收集的特定挥发性有机化合物（VOC）的鉴定和苦果豆的分子分化非常重要。方法采用中性解吸萃取大气压化学电离质谱法（ND-EAPCI-MS）鉴定了十种不同苦参品种花中释放的二十八种挥发性有机化合物，而没有进行任何化学预处理或收集任何挥发性有机化合物。通过高分辨率MSn分析进行化学鉴定，并在可能的情况下通过分析标准液进行确认。结果根据我们的文献检索，鉴定出了九种挥发性有机化合物，3-丁烯-2-一，环己二烯，2-甲基呋喃，3-烯丙基环己烯，枯基乙醇，氧化三烯醇氧化物，环氧-芳樟醇氧化物，N-（2-羟乙基）辛酰胺早先的研究中尚未报道过香茅中的3-羟基-二氢-β-紫罗兰酮和3-羟基二氢-β-紫罗兰酮。通过质谱分析结果的主成分分析，可以可靠地区分10个不同种类的草莓香豆草。结论我们的ND-EAPCI-MS分析结果大大增加了我们对O的化学知识。