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Metagenomics: Is it a powerful tool to obtain lipases for application in biocatalysis?
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ( IF 3.2 ) Pub Date : 2019-11-20 , DOI: 10.1016/j.bbapap.2019.140320 Janaina Marques Almeida 1 , Robson Carlos Alnoch 1 , Emanuel Maltempi Souza 1 , David Alexander Mitchell 1 , Nadia Krieger 2
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ( IF 3.2 ) Pub Date : 2019-11-20 , DOI: 10.1016/j.bbapap.2019.140320 Janaina Marques Almeida 1 , Robson Carlos Alnoch 1 , Emanuel Maltempi Souza 1 , David Alexander Mitchell 1 , Nadia Krieger 2
Affiliation
In recent years, metagenomic strategies have been widely used to isolate and identify new enzymes from uncultivable components of microbial communities. Among these enzymes, various lipases have been obtained from metagenomic libraries from different environments and characterized. Although many of these lipases have characteristics that could make them interesting for application in biocatalysis, relatively little work has been done to evaluate their potential to catalyze industrially important reactions. In the present article, we highlight the latest research on lipases obtained through metagenomic tools, focusing on studies of activity and stability and investigations of application in biocatalysis. We also discuss the challenges of metagenomic approaches for the bioprospecting of new lipases.
中文翻译:
元基因组学:它是获得用于生物催化的脂肪酶的有力工具吗?
近年来,宏基因组学策略已被广泛用于从微生物群落不可培养的成分中分离和鉴定新酶。在这些酶中,已经从来自不同环境的宏基因组文库中获得了各种脂肪酶并进行了表征。尽管这些脂肪酶中的许多酶具有使其在生物催化中有趣的特性,但评估其催化工业上重要反应的潜力的工作相对较少。在本文中,我们重点介绍通过宏基因组学工具获得的脂肪酶的最新研究,重点是活性和稳定性的研究以及在生物催化中的应用研究。我们还将讨论宏基因组学方法对新脂肪酶的生物勘探的挑战。
更新日期:2019-11-20
中文翻译:
元基因组学:它是获得用于生物催化的脂肪酶的有力工具吗?
近年来,宏基因组学策略已被广泛用于从微生物群落不可培养的成分中分离和鉴定新酶。在这些酶中,已经从来自不同环境的宏基因组文库中获得了各种脂肪酶并进行了表征。尽管这些脂肪酶中的许多酶具有使其在生物催化中有趣的特性,但评估其催化工业上重要反应的潜力的工作相对较少。在本文中,我们重点介绍通过宏基因组学工具获得的脂肪酶的最新研究,重点是活性和稳定性的研究以及在生物催化中的应用研究。我们还将讨论宏基因组学方法对新脂肪酶的生物勘探的挑战。