Effectors secreted by microbes contribute to pathogen virulence and/or avirulence on host plants in the interaction of plants and microbes. Also, the effector repertoire determines the host specificity of a pathogen. Fusarium oxysporum f. sp. cubense (Foc) is the causal agent of banana wilt; however, knowledge about Foc effector genes is very limited. In this study, genome-wide effector gene identification was performed in Foc race 1 (Foc 1) and Foc race 4 (Foc 4) based on the context association between the effector genes and the transposable element mimp. A total of 20 candidate effector genes were identified, of which 3 were Foc 1-specific, 6 were Foc 4-specific, and 11 were present in both Foc 1 and Foc 4. Most genes (14 out of 20) showed a significant transcriptional burst in planta compared with in-culture conditions, from more than 10-fold to 1,617-fold, and at the highest 32,725-fold. In addition to Foc 1- and Foc 4-specific genes, the genes Foc 283, Foc 495, and Foc 594 also exhibited transcriptional race specificity. Sixteen of the twenty genes were predicted to have a signal peptide, nine genes might encode real effectors predicted by EffectorP 2.0, and eight genes had predicted motifs. To validate the pathogenicity of the candidate effector genes, we generated knockout mutant and complementants of the gene Foc 1324 and tested their virulence on banana plants. The results showed that Foc 1324 was a virulent factor and required for the pathogenicity of Foc 4.

微生物分泌的效应子在植物和微生物的相互作用中有助于病原体的致病性和/或宿主植物上的无毒性。同样，效应子库决定了病原体的宿主特异性。尖孢镰刀菌f。sp。香蕉枯萎病（FOC）是香蕉枯萎病的致病原; 但是，关于Foc效应子基因的知识非常有限。在这项研究中，全基因组效应基因鉴定进行FOC种族1（FOC 1）和FOC种族4（FOC基于所述效应基因和转座元件之间的上下文关联4）MIMP。总共鉴定出20个候选效应基因，其中3个是Foc 1特异性的，6个是Foc 4特异性的，在Foc 1和Foc 4中都存在11个。大多数基因（20个中的14个）显示出显着的转录与培养条件相比，植物的爆裂率从10倍以上增加到1617倍，最高达到32725倍。除了Foc 1和Foc 4特异性基因外，基因Foc 283，Foc 495和Foc 594还表现出转录种族特异性。预计二十个基因中的十六个具有信号肽，九个基因可能编码由EffectorP 2.0预测的真实效应子，八个基因具有预测的基序。为了验证候选效应基因的致病性，我们生成了基因Foc 1324的敲除突变体和互补体，并测试了它们对香蕉植物的毒力。结果表明Foc 1324是一种致病因子，是Foc 4致病性所必需的。