Roles of mitochondrial Na+-Ca2+ exchanger, NCLX, were studied in B lymphocytes such as heterozygous NCLX knockout DT40 cells, NCLX knockdown A20 cells, and native mouse spleen B lymphocytes treated with a NCLX blocker, CGP-37157. Cytosolic Ca2+ response to B cell receptor stimulation was impaired in these B lymphocytes, demonstrating importance of mitochondria-ER Ca2+ recycling via NCLX and sarco/endoplasmic reticulum Ca2+-ATPase SERCA, and interaction with store-operated Ca2+ entry. NCLX was also associated with motility and chemotaxis of B lymphocyte. Contrary to B lymphocytes, contribution of NCLX in mouse spleen T lymphocytes was minor.

中文翻译：

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线粒体Na + -Ca2 +交换子NCLX在淋巴细胞中的生理功能。

研究了线粒体Na + -Ca2 +交换子NCLX在B淋巴细胞中的作用，例如杂合的NCLX敲除DT40细胞，NCLX敲除的A20细胞以及用NCLX阻滞剂CGP-37157处理的天然小鼠脾脏B淋巴细胞。这些B淋巴细胞对B细胞受体刺激的胞质Ca2 +响应受到损害，表明通过NCLX和肌浆网/内质网Ca2 + -ATPase SERCA回收线粒体-ER Ca2 +的重要性，以及与储存操作的Ca2 +进入的相互作用。NCLX还与B淋巴细胞的运动性和趋化性有关。与B淋巴细胞相反，NCLX在小鼠脾T淋巴细胞中的贡献很小。