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Time-resolved serial femtosecond crystallography at the European XFEL.
Nature Methods ( IF 48.0 ) Pub Date : 2019-11-18 , DOI: 10.1038/s41592-019-0628-z
Suraj Pandey 1 , Richard Bean 2 , Tokushi Sato 2 , Ishwor Poudyal 1 , Johan Bielecki 2 , Jorvani Cruz Villarreal 3 , Oleksandr Yefanov 4 , Valerio Mariani 4 , Thomas A White 4 , Christopher Kupitz 5 , Mark Hunter 5 , Mohamed H Abdellatif 4 , Saša Bajt 6 , Valerii Bondar 2 , Austin Echelmeier 3 , Diandra Doppler 3 , Moritz Emons 2 , Matthias Frank 7 , Raimund Fromme 3 , Yaroslav Gevorkov 4, 8 , Gabriele Giovanetti 2 , Man Jiang 2 , Daihyun Kim 3 , Yoonhee Kim 2 , Henry Kirkwood 2 , Anna Klimovskaia 2 , Juraj Knoska 4, 9 , Faisal H M Koua 4 , Romain Letrun 2 , Stella Lisova 10 , Luis Maia 2 , Victoria Mazalova 4 , Domingo Meza 11 , Thomas Michelat 2 , Abbas Ourmazd 1 , Guido Palmer 2 , Marco Ramilli 2 , Robin Schubert 11 , Peter Schwander 1 , Alessandro Silenzi 2 , Jolanta Sztuk-Dambietz 2 , Alexandra Tolstikova 4 , Henry N Chapman 4, 9, 12 , Alexandra Ros 3 , Anton Barty 4 , Petra Fromme 3 , Adrian P Mancuso 2, 13 , Marius Schmidt 1
Affiliation  

The European XFEL (EuXFEL) is a 3.4-km long X-ray source, which produces femtosecond, ultrabrilliant and spatially coherent X-ray pulses at megahertz (MHz) repetition rates. This X-ray source has been designed to enable the observation of ultrafast processes with near-atomic spatial resolution. Time-resolved crystallographic investigations on biological macromolecules belong to an important class of experiments that explore fundamental and functional structural displacements in these molecules. Due to the unusual MHz X-ray pulse structure at the EuXFEL, these experiments are challenging. Here, we demonstrate how a biological reaction can be followed on ultrafast timescales at the EuXFEL. We investigate the picosecond time range in the photocycle of photoactive yellow protein (PYP) with MHz X-ray pulse rates. We show that difference electron density maps of excellent quality can be obtained. The results connect the previously explored femtosecond PYP dynamics to timescales accessible at synchrotrons. This opens the door to a wide range of time-resolved studies at the EuXFEL.

中文翻译:

欧洲 XFEL 的时间分辨系列飞秒晶体学。

欧洲 XFEL (EuXFEL) 是一个 3.4 公里长的 X 射线源,它以兆赫兹 (MHz) 的重复频率产生飞秒、超亮和空间相干 X 射线脉冲。该 X 射线源旨在以近原子空间分辨率观察超快过程。对生物大分子的时间分辨晶体学研究属于一类重要的实验,旨在探索这些分子中的基本和功能结构位移。由于 EuXFEL 上不寻常的 MHz X 射线脉冲结构,这些实验具有挑战性。在这里,我们展示了如何在 EuXFEL 的超快时间尺度上跟踪生物反应。我们研究了具有 MHz X 射线脉冲速率的光敏黄色蛋白 (PYP) 光循环中的皮秒时间范围。我们表明可以获得质量优异的不同电子密度图。结果将先前探索的飞秒 PYP 动力学与同步加速器可访问的时间尺度联系起来。这为在 EuXFEL 进行广泛的时间分辨研究打开了大门。
更新日期:2019-11-18
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